PUBLICATION

Novel hypophysiotropic AgRP2 neurons and pineal cells revealed by BAC transgenesis in zebrafish

Authors
Shainer, I., Buchshtab, A., Hawkins, T.A., Wilson, S.W., Cone, R.D., Gothilf, Y.
ID
ZDB-PUB-170321-9
Date
2017
Source
Scientific Reports   7: 44777 (Journal)
Registered Authors
Cone, Roger, Gothilf, Yoav, Hawkins, Tom, Wilson, Steve
Keywords
Hypothalamus, Neuroendocrinology
MeSH Terms
  • Neurons/metabolism*
  • Agouti-Related Protein/metabolism*
  • Cell Communication
  • Gene Transfer Techniques*
  • Animals
  • Pineal Gland/cytology*
  • Preoptic Area/metabolism
  • Zebrafish/genetics*
  • Animals, Genetically Modified
  • Chromosomes, Artificial, Bacterial/genetics*
  • Pituitary Gland/metabolism*
(all 11)
PubMed
28317906 Full text @ Sci. Rep.
Abstract
The neuropeptide agouti-related protein (AgRP) is expressed in the arcuate nucleus of the mammalian hypothalamus and plays a key role in regulating food consumption and energy homeostasis. Fish express two agrp genes in the brain: agrp1, considered functionally homologous with the mammalian AgRP, and agrp2. The role of agrp2 and its relationship to agrp1 are not fully understood. Utilizing BAC transgenesis, we generated transgenic zebrafish in which agrp1- and agrp2-expressing cells can be visualized and manipulated. By characterizing these transgenic lines, we showed that agrp1-expressing neurons are located in the ventral periventricular hypothalamus (the equivalent of the mammalian arcuate nucleus), projecting throughout the hypothalamus and towards the preoptic area. The agrp2 gene was expressed in the pineal gland in a previously uncharacterized subgroup of cells. Additionally, agrp2 was expressed in a small group of neurons in the preoptic area that project directly towards the pituitary and form an interface with the pituitary vasculature, suggesting that preoptic AgRP2 neurons are hypophysiotropic. We showed that direct synaptic connection can exist between AgRP1 and AgRP2 neurons in the hypothalamus, suggesting communication and coordination between AgRP1 and AgRP2 neurons and, therefore, probably also between the processes they regulate.
Genes / Markers
Figures
Figure Gallery (6 images)
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Expression
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay Figure
agrpWTcontrolDay 6ISH
asip2bWTstandard conditionsAdultRTPCR
asip2bWTstandard conditionsAdultRTPCR
asip2bWTcontrolDay 6ISH
asip2bWTstandard conditionsAdultNot DetectedRTPCR
asip2bWTcontrolDay 6ISH
EGFPbiu5Tg; c264Tg; tlv05TgcontrolDays 7-13IFL
EGFPc264Tg; s843Tg; tlv05TgcontrolDays 7-13IFL
EGFPc264Tg; s843Tg; tlv05TgcontrolDays 30-44IFL
EGFPc264Tg; s843Tg; tlv05TgcontrolDays 7-13IFL
1 - 10 of 56
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Phenotype
No data available
Mutations / Transgenics
Allele Construct Type Affected Genomic Region
biu5TgTransgenic Insertion
    c264TgTransgenic Insertion
      s843TgTransgenic Insertion
        tlv04TgTransgenic Insertion
          tlv05TgTransgenic Insertion
            tlv06TgTransgenic Insertion
              wz01TgTransgenic Insertion
                y9TgTransgenic Insertion
                  zf44TgTransgenic Insertion
                    zf104TgTransgenic Insertion
                      1 - 10 of 10
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                      Human Disease / Model
                      No data available
                      Sequence Targeting Reagents
                      No data available
                      Fish
                      Fish
                      biu5Tg; c264Tg; tlv05Tg
                      c264Tg; s843Tg; tlv05Tg
                      c264Tg; tlv04Tg
                      c264Tg; tlv04Tg; tlv06Tg
                      c264Tg; tlv04Tg; zf44Tg
                      c264Tg; tlv05Tg
                      c264Tg; tlv05Tg; wz01Tg
                      c264Tg; tlv05Tg; y9Tg
                      c264Tg; tlv05Tg; zf44Tg
                      c264Tg; tlv05Tg; zf104Tg
                      1 - 10 of 12
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                      Antibodies
                      Orthology
                      No data available
                      Engineered Foreign Genes
                      Marker Marker Type Name
                      EGFPEFGEGFP
                      GAL4EFGGAL4
                      GFPEFGGFP
                      mCherryEFGmCherry
                      NTREFGNTR
                      1 - 5 of 5
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                      Mapping
                      No data available
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                      Citation
                      Shainer, I., Buchshtab, A., Hawkins, T.A., Wilson, S.W., Cone, R.D., Gothilf, Y. (2017) Novel hypophysiotropic AgRP2 neurons and pineal cells revealed by BAC transgenesis in zebrafish. Scientific Reports. 7:44777.