PUBLICATION

The enteric nervous system promotes intestinal health by constraining microbiota composition

Authors
Rolig, A.S., Mittge, E.K., Ganz, J., Troll, J.V., Melancon, E., Wiles, T.J., Alligood, K., Stephens, W.Z., Eisen, J.S., Guillemin, K.
ID
ZDB-PUB-170217-2
Date
2017
Source
PLoS Biology   15: e2000689 (Journal)
Registered Authors
Eisen, Judith S., Ganz, Julia, Guillemin, Karen, Mittge, Erika K.
Keywords
none
MeSH Terms
  • Bacteria/growth & development
  • Bacteria/isolation & purification
  • Cell Count
  • Enteric Nervous System/cytology
  • Enteric Nervous System/physiology*
  • Zebrafish Proteins/metabolism
  • Neutrophils/metabolism
  • SOXE Transcription Factors/metabolism
  • Colony Count, Microbial
  • Phylogeny
  • Intestines/microbiology*
  • Intestines/pathology
  • Zebrafish
  • Leukocyte Count
  • Dysbiosis/genetics
  • Dysbiosis/microbiology
  • Dysbiosis/pathology
  • Inflammation/genetics
  • Inflammation/pathology
  • Stem Cell Transplantation
  • Animals
  • Mutation/genetics
  • Models, Biological
  • Gastrointestinal Microbiome*
  • Gene Expression Regulation
(all 25)
PubMed
28207737 Full text @ PLoS Biol.
Abstract
Sustaining a balanced intestinal microbial community is critical for maintaining intestinal health and preventing chronic inflammation. The gut is a highly dynamic environment, subject to periodic waves of peristaltic activity. We hypothesized that this dynamic environment is a prerequisite for a balanced microbial community and that the enteric nervous system (ENS), a chief regulator of physiological processes within the gut, profoundly influences gut microbiota composition. We found that zebrafish lacking an ENS due to a mutation in the Hirschsprung disease gene, sox10, develop microbiota-dependent inflammation that is transmissible between hosts. Profiling microbial communities across a spectrum of inflammatory phenotypes revealed that increased levels of inflammation were linked to an overabundance of pro-inflammatory bacterial lineages and a lack of anti-inflammatory bacterial lineages. Moreover, either administering a representative anti-inflammatory strain or restoring ENS function corrected the pathology. Thus, we demonstrate that the ENS modulates gut microbiota community membership to maintain intestinal health.
Genes / Markers
Figures
Figure Gallery (3 images)
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Expression
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay Figure
saasox10t3/t3; i114TgcontrolDay 6RTPCR
1 - 1 of 1
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Phenotype
Mutations / Transgenics
Allele Construct Type Affected Genomic Region
i114TgTransgenic Insertion
    t3
      Insertion
      1 - 2 of 2
      Show
      Human Disease / Model
      Human Disease Fish Conditions Evidence
      Hirschsprung's diseasesox10t3/t3controlTAS
      1 - 1 of 1
      Show
      Sequence Targeting Reagents
      Target Reagent Reagent Type
      alpi.1MO1-alpi.1MRPHLNO
      tnfrsf1aMO1-tnfrsf1aMRPHLNO
      tnfrsf1aMO2-tnfrsf1aMRPHLNO
      1 - 3 of 3
      Show
      Fish
      Antibodies
      No data available
      Orthology
      No data available
      Engineered Foreign Genes
      Marker Marker Type Name
      GFPEFGGFP
      1 - 1 of 1
      Show
      Mapping
      No data available