PUBLICATION

Embryonic origin and lineage hierarchies of the neural progenitor subtypes building the zebrafish adult midbrain

Authors
Galant, S., Furlan, G., Coolen, M., Dirian, L., Foucher, I., Bally-Cuif, L.
ID
ZDB-PUB-161007-38
Date
2016
Source
Developmental Biology   420(1): 120-135 (Journal)
Registered Authors
Bally-Cuif, Laure, Coolen, Marion, Dirian, Lara, Foucher, Isabelle
Keywords
Neuroepithelial progenitor, Optic tectum, Pallium, Post-embryonic neurogenesis, Radial glia, Zebrafish
MeSH Terms
  • Embryo, Nonmammalian/cytology
  • Embryo, Nonmammalian/drug effects
  • Embryo, Nonmammalian/metabolism
  • Cell Lineage*/drug effects
  • Superior Colliculi/cytology
  • Superior Colliculi/drug effects
  • Superior Colliculi/embryology
  • Superior Colliculi/metabolism
  • Neuroepithelial Cells/cytology
  • Neuroepithelial Cells/drug effects
  • Neuroepithelial Cells/metabolism
  • Animals
  • Zebrafish/embryology*
  • Mesencephalon/cytology
  • Mesencephalon/drug effects
  • Mesencephalon/embryology*
  • Neural Stem Cells/cytology*
  • Neural Stem Cells/drug effects
  • Neural Stem Cells/metabolism
  • Neurons/cytology
  • Neurons/drug effects
  • Neurons/metabolism
  • Recombination, Genetic/genetics
  • Neurogenesis/drug effects
  • Doxycycline/pharmacology
  • Tamoxifen/analogs & derivatives
  • Tamoxifen/pharmacology
  • Aging/physiology*
  • Neuroglia/cytology
  • Neuroglia/drug effects
  • Neuroglia/metabolism
(all 31)
PubMed
27693369 Full text @ Dev. Biol.
Abstract
Neurogenesis in the post-embryonic vertebrate brain varies in extent and efficiency between species and brain territories. Distinct neurogenesis modes may account for this diversity, and several neural progenitor subtypes, radial glial cells (RG) and neuroepithelial progenitors (NE), have been identified in the adult zebrafish brain. The neurogenic sequences issued from these progenitors, and their contribution to brain construction, remain incompletely understood. Here we use genetic tracing techniques based on conditional Cre recombination and Tet-On neuronal birthdating to unravel the neurogenic sequence operating from NE progenitors in the zebrafish post-embryonic optic tectum. We reveal that a subpopulation of her5-positive NE cells of the posterior midbrain layer stands at the top of a neurogenic hierarchy involving, in order, the amplification pool of the tectal proliferation zone (TPZ), followed by her4-positive RG cells with transient neurogenic activity. We further demonstrate that the adult her5-positive NE pool is issued in lineage from an identically located NE pool expressing the same gene in the embryonic neural tube. Finally, we show that these features are reminiscent of the neurogenic sequence and embryonic origin of the her9-positive progenitor NE pool involved in the construction of the lateral pallium at post-embryonic stages. Together, our results highlight the shared recruitment of an identical neurogenic strategy by two remote brain territories, where long-lasting NE pools serve both as a growth zone and as the life-long source of young neurogenic RG cells.
Genes / Markers
Figures
Figure Gallery (11 images) / 2
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Expression
Phenotype
No data available
Mutations / Transgenics
Allele Construct Type Affected Genomic Region
gy3TgTransgenic Insertion
    gy4TgTransgenic Insertion
      ip3TgTransgenic Insertion
        ip4TgTransgenic Insertion
          jh15TgTransgenic Insertion
            knu2TgTransgenic Insertion
              mi2001TgTransgenic Insertion
                ne1939TgTransgenic Insertion
                  vu298aTgTransgenic Insertion
                    y83TgTransgenic Insertion
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                      Human Disease / Model
                      No data available
                      Sequence Targeting Reagents
                      No data available
                      Fish
                      No data available
                      Antibodies
                      Orthology
                      No data available
                      Engineered Foreign Genes
                      Marker Marker Type Name
                      CreEFGCre
                      EGFPEFGEGFP
                      GFPEFGGFP
                      mCherryEFGmCherry
                      RFPEFGRFP
                      VenusEFGVenus
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                      Mapping
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                      Citation
                      Galant, S., Furlan, G., Coolen, M., Dirian, L., Foucher, I., Bally-Cuif, L. (2016) Embryonic origin and lineage hierarchies of the neural progenitor subtypes building the zebrafish adult midbrain. Developmental Biology. 420(1):120-135.