PUBLICATION

Microglia Colonization of Developing Zebrafish Midbrain Is Promoted by Apoptotic Neuron and Lysophosphatidylcholine

Authors
Xu, J., Wang, T., Wu, Y., Jin, W., Wen, Z.
ID
ZDB-PUB-160720-10
Date
2016
Source
Developmental Cell   38(2): 214-22 (Journal)
Registered Authors
Wen, Zilong
Keywords
none
MeSH Terms
  • Animals
  • Zebrafish Proteins/genetics
  • Zebrafish Proteins/metabolism
  • Microglia/cytology
  • Microglia/physiology*
  • Neurogenesis/drug effects
  • Apoptosis*
  • Brain/cytology*
  • Brain/drug effects
  • Brain/physiology
  • Neurons/drug effects
  • Neurons/metabolism
  • Neurons/pathology*
  • Zebrafish/genetics
  • Zebrafish/growth & development*
  • Zebrafish/metabolism
  • Lysophosphatidylcholines/pharmacology*
  • Cell Differentiation
(all 18)
PubMed
27424497 Full text @ Dev. Cell
Abstract
Microglia are CNS-resident macrophages and play important roles in neural development and function. However, how microglial precursors born in peripheral tissues colonize the CNS remains undefined. Using in vivo imaging and genetic manipulation of zebrafish, we showed that microglial precursors enter the optic tectum of the midbrain, where the majority of microglia reside during early development, via the lateral periphery between the eyes and brain and the ventral periphery of the brain in a circulation-independent manner. The colonization of the optic tectum by microglial precursors is dynamic and driven by apoptotic neuronal death, which occurs naturally in the midbrain during neurogenesis. We further show that lysophosphatidylcholine, a phospholipid known to be released from apoptotic cells, can promote microglial precursor entry into the brain via its cognate receptors grp132b. Our study reveals that microglia colonization of developing zebrafish midbrain is triggered by apoptotic neuronal death, possibly via releasing lysophosphatidylcholine.
Genes / Markers
Figures
Figure Gallery (8 images)
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Expression
Phenotype
Mutations / Transgenics
Allele Construct Type Affected Genomic Region
hkz011tTgTransgenic Insertion
    hkz013tTgTransgenic Insertion
      hkz014tTgTransgenic Insertion
        hkz015tTgTransgenic Insertion
          nz50TgTransgenic Insertion
            s843TgTransgenic Insertion
              tsu11TgTransgenic Insertion
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                Human Disease / Model
                No data available
                Sequence Targeting Reagents
                Target Reagent Reagent Type
                gpr132aMO1-gpr132aMRPHLNO
                gpr132bMO1-gpr132bMRPHLNO
                tnnt2aMO1-tnnt2aMRPHLNO
                1 - 3 of 3
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                Fish
                Antibodies
                No data available
                Orthology
                No data available
                Engineered Foreign Genes
                Marker Marker Type Name
                DsRed2EFGDsRed2
                DsRedxEFGDsRedx
                EGFPEFGEGFP
                KaedeEFGKaede
                1 - 4 of 4
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                Mapping
                No data available