PUBLICATION
BMP and retinoic acid regulate anterior-posterior patterning of the non-axial mesoderm across the dorsal-ventral axis
- Authors
- Naylor, R.W., Skvarca, L.B., Thisse, C., Thisse, B., Hukriede, N.A., Davidson, A.J.
- ID
- ZDB-PUB-160715-7
- Date
- 2016
- Source
- Nature communications 7: 12197 (Journal)
- Registered Authors
- Davidson, Alan, Hukriede, Neil, Naylor, Richard, Thisse, Bernard, Thisse, Christine
- Keywords
- none
- MeSH Terms
-
- Bone Morphogenetic Proteins/genetics
- Retinoic Acid 4-Hydroxylase/genetics*
- Zebrafish Proteins/genetics*
- Kidney/embryology*
- Tretinoin/metabolism*
- Tretinoin/pharmacology
- Zebrafish/embryology*
- Body Patterning/drug effects
- Body Patterning/genetics*
- Bone Morphogenetic Protein 2/genetics*
- Animals
- Embryo, Nonmammalian
- Mesoderm/embryology*
- PubMed
- 27406002 Full text @ Nat. Commun.
Citation
Naylor, R.W., Skvarca, L.B., Thisse, C., Thisse, B., Hukriede, N.A., Davidson, A.J. (2016) BMP and retinoic acid regulate anterior-posterior patterning of the non-axial mesoderm across the dorsal-ventral axis. Nature communications. 7:12197.
Abstract
Despite the fundamental importance of patterning along the dorsal-ventral (DV) and anterior-posterior (AP) axes during embryogenesis, uncertainty exists in the orientation of these axes for the mesoderm. Here we examine the origin and formation of the zebrafish kidney, a ventrolateral mesoderm derivative, and show that AP patterning of the non-axial mesoderm occurs across the classic gastrula stage DV axis while DV patterning aligns along the animal-vegetal pole. We find that BMP signalling acts early to establish broad anterior and posterior territories in the non-axial mesoderm while retinoic acid (RA) functions later, but also across the classic DV axis. Our data support a model in which RA on the dorsal side of the embryo induces anterior kidney fates while posterior kidney progenitors are protected ventrally by the RA-catabolizing enzyme Cyp26a1. This work clarifies our understanding of vertebrate axis orientation and establishes a new paradigm for how the kidney and other mesodermal derivatives arise during embryogenesis.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping