PUBLICATION

BMP and retinoic acid regulate anterior-posterior patterning of the non-axial mesoderm across the dorsal-ventral axis

Authors

Naylor, R.W., Skvarca, L.B., Thisse, C., Thisse, B., Hukriede, N.A., Davidson, A.J.

ID

ZDB-PUB-160715-7

Date

2016

Source

Nature communications 7: 12197 (Journal)

Registered Authors

Davidson, Alan, Hukriede, Neil, Naylor, Richard, Thisse, Bernard, Thisse, Christine

Keywords

none

MeSH Terms

Zebrafish Proteins/genetics*
Retinoic Acid 4-Hydroxylase/genetics*
Body Patterning/drug effects
Body Patterning/genetics*
Animals
Mesoderm/embryology*
Tretinoin/metabolism*
Tretinoin/pharmacology
Bone Morphogenetic Protein 2/genetics*
Embryo, Nonmammalian
Kidney/embryology*
Zebrafish/embryology*
Bone Morphogenetic Proteins/genetics

(all 13)

PubMed

27406002 Full text @ Nat. Commun.

Abstract

Despite the fundamental importance of patterning along the dorsal-ventral (DV) and anterior-posterior (AP) axes during embryogenesis, uncertainty exists in the orientation of these axes for the mesoderm. Here we examine the origin and formation of the zebrafish kidney, a ventrolateral mesoderm derivative, and show that AP patterning of the non-axial mesoderm occurs across the classic gastrula stage DV axis while DV patterning aligns along the animal-vegetal pole. We find that BMP signalling acts early to establish broad anterior and posterior territories in the non-axial mesoderm while retinoic acid (RA) functions later, but also across the classic DV axis. Our data support a model in which RA on the dorsal side of the embryo induces anterior kidney fates while posterior kidney progenitors are protected ventrally by the RA-catabolizing enzyme Cyp26a1. This work clarifies our understanding of vertebrate axis orientation and establishes a new paradigm for how the kidney and other mesodermal derivatives arise during embryogenesis.

Genes / Markers

Figures

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Expression

Gene	Fish	Conditions	Stage	Anatomy	Assay	Figure
aldh1a2	TU	chemical treatment by environment: dorsomorphin	75%-epiboly	mesoderm	ISH	Fig. 6
aldh1a2	TU	control	75%-epiboly	mesoderm	ISH	Fig. 6
aldh1a2	TU	standard conditions	75%-epiboly	mesoderm	ISH	Fig. 2
bmp4	TU	standard conditions	75%-epiboly	epiblast	ISH	Fig. 2
cyp26a1	TU	standard conditions	75%-epiboly	mesoderm	ISH	Fig. 2
cyp26a1	TU	chemical treatment by environment: dorsomorphin	75%-epiboly	mesoderm	ISH	Fig. 6
cyp26a1	TU	control	75%-epiboly	mesoderm	ISH	Fig. 6
fsta	TU	standard conditions	75%-epiboly	mesoderm	ISH	Fig. 2
gata1a	TU	standard conditions	10-13 somites	intermediate mesoderm	ISH	Fig. 3
gata1a	TU	standard conditions	10-13 somites	presumptive blood	ISH	Fig. 3

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Phenotype

Fish	Conditions	Stage	Phenotype	Figure
TU	chemical treatment by environment: 4-(diethylamino)benzaldehyde	14-19 somites	pronephric distal late tubule slc12a3 expression increased distribution, abnormal	Fig. 7
TU	chemical treatment by environment: 4-(diethylamino)benzaldehyde	14-19 somites	pronephric proximal convoluted tubule slc4a4a expression absent, abnormal	Fig. 7
TU	chemical treatment by environment: 4-(diethylamino)benzaldehyde	14-19 somites	pronephric proximal straight tubule slc4a4a expression absent, abnormal	Fig. 7
TU	chemical treatment by environment: 4-(diethylamino)benzaldehyde	Prim-5	pronephric distal early tubule slc12a1 expression absent, abnormal	Fig. 1
TU	chemical treatment by environment: 4-(diethylamino)benzaldehyde	Prim-5	pronephric distal late tubule slc12a3 expression increased distribution, abnormal	Fig. 1
TU	chemical treatment by environment: 4-(diethylamino)benzaldehyde	Prim-5	pronephric proximal convoluted tubule slc4a4a expression absent, abnormal	Fig. 1
TU	chemical treatment by environment: 4-(diethylamino)benzaldehyde	Prim-5	pronephric proximal straight tubule slc4a4a expression absent, abnormal	Fig. 1
TU	chemical treatment by environment: retinoic acid, chemical treatment by environment: 4-(diethylamino)benzaldehyde	14-19 somites	pronephric distal late tubule slc12a3 expression absent, abnormal	Fig. 7
TU	chemical treatment by environment: dorsomorphin, chemical treatment by environment: 4-(diethylamino)benzaldehyde	14-19 somites	pronephric distal late tubule slc12a3 expression increased distribution, abnormal	Fig. 7
TU	chemical treatment by environment: dorsomorphin, chemical treatment by environment: 4-(diethylamino)benzaldehyde	14-19 somites	pronephric proximal convoluted tubule slc4a4a expression absent, abnormal	Fig. 7

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Mutations / Transgenics

Human Disease / Model

Sequence Targeting Reagents

Fish

Antibodies

Orthology

Engineered Foreign Genes

Mapping

Naylor et al., 2016 ZDB-PUB-160715-7 PMID:27406002

BMP and retinoic acid regulate anterior-posterior patterning of the non-axial mesoderm across the dorsal-ventral axis

Naylor et al., 2016

ZDB-PUB-160715-7
PMID:27406002