PUBLICATION

Wnt/β-catenin signaling promotes regeneration after adult zebrafish Spinal Cord injury

Authors
Strand, N.S., Hoi, K.K., Phan, T.M., Ray, C.A., Berndt, J.D., Moon, R.T.
ID
ZDB-PUB-160709-13
Date
2016
Source
Biochemical and Biophysical Research Communications   477(4): 952-6 (Journal)
Registered Authors
Berndt, Jason, Moon, Randall T., Strand, Nicholas
Keywords
Regeneration, Spinal cord injury, Wnt/β-catenin signaling, Zebrafish
MeSH Terms
  • Wnt Signaling Pathway*
  • Animals
  • Up-Regulation
  • Spinal Cord Regeneration/physiology*
  • Spinal Cord Injuries/pathology
  • Spinal Cord Injuries/physiopathology*
  • beta Catenin/metabolism*
  • Spinal Cord/pathology
  • Spinal Cord/physiopathology*
  • Zebrafish/anatomy & histology
  • Zebrafish/physiology*
(all 11)
PubMed
27387232 Full text @ Biochem. Biophys. Res. Commun.
Abstract
Unlike mammals, zebrafish can regenerate injured spinal cord and regain control of caudal tissues. It was recently shown that Wnt/β-catenin signaling is necessary for spinal cord regeneration in the larval zebrafish. However, the molecular mechanisms of regeneration may or may not be conserved between larval and adult zebrafish. To test this, we assessed the role of Wnt/β-catenin signaling after spinal cord injury in the adult zebrafish. We show that Wnt/β-catenin signaling is increased after spinal cord injury in the adult zebrafish. Moreover, overexpression of Dkk1b inhibited Wnt/β-catenin signaling in the regenerating spinal cord of adult zebrafish. Dkk1b overexpression also inhibited locomotor recovery, axon regeneration, and glial bridge formation in the injured spinal cord. Thus, our data illustrate a conserved role for Wnt/β-catenin signaling in adult and larval zebrafish spinal cord regeneration.
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Mutations / Transgenics
Allele Construct Type Affected Genomic Region
ia5TgTransgenic Insertion
    w32TgTransgenic Insertion
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      Engineered Foreign Genes
      Marker Marker Type Name
      GFPEFGGFP
      mCherryEFGmCherry
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