PUBLICATION

RNA polymerase III component Rpc9 regulates hematopoietic stem and progenitor cell maintenance in zebrafish

Authors
Wei, Y., Xu, J., Zhang, W., Wen, Z., Liu, F.
ID
ZDB-PUB-160507-8
Date
2016
Source
Development (Cambridge, England)   143(12): 2103-10 (Journal)
Registered Authors
Liu, Feng, Wei, Yonglong, Wen, Zilong
Keywords
RNA polymerase III, Rpc9, P53, Hematopoietic stem and progenitor cells, Zebrafish
MeSH Terms
  • Amino Acid Sequence
  • RNA Polymerase III/metabolism*
  • Cell Proliferation
  • Gene Knockdown Techniques
  • Zebrafish Proteins/metabolism*
  • Cell Survival
  • Phenotype
  • Hematopoietic Stem Cells/cytology*
  • Hematopoietic Stem Cells/metabolism*
  • Embryo, Nonmammalian/metabolism
  • Hematopoiesis
  • Zebrafish/metabolism*
  • Animals
  • Base Sequence
  • Mutation/genetics
  • T-Lymphocytes/cytology
  • Tumor Suppressor Protein p53/metabolism
(all 17)
PubMed
27151951 Full text @ Development
Abstract
Hematopoietic stem and progenitor cells (HSPCs) are capable of self-renewal and replenishing all lineages of blood cells throughout the lifetime and thus critical for tissue homeostasis. However, the mechanism regulating HSPC development is still incompletely understood. Here, we isolate a zebrafish mutant with defective T lymphopoiesis and positional cloning identifies that Rpc9, a component of DNA-directed RNA polymerase III (Pol III) complex, is responsible for the mutant phenotype. Further analysis shows that rpc9-deficiency leads to the impairment of HSPCs and their derivatives in zebrafish embryos. Excessive apoptosis is observed in the caudal hematopoietic tissue (CHT, the equivalent of fetal liver in mammals) of rpc9(-/-) embryos and the hematopoietic defects in rpc9(-/-) embryos can be fully rescued by suppression of p53 Thus, our work illustrate that Rpc9, a component of Pol III, plays an important tissue-specific role in HSPC maintenance during zebrafish embryogenesis and that it might be conserved across vertebrates including mammals.
Genes / Markers
Figures
Figure Gallery (12 images) / 2
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Expression
Phenotype
Mutations / Transgenics
Allele Construct Type Affected Genomic Region
hkz8
    Point Mutation
    zdf1
      Point Mutation
      1 - 2 of 2
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      Human Disease / Model
      No data available
      Sequence Targeting Reagents
      Target Reagent Reagent Type
      crcpMO1-crcpMRPHLNO
      polr3hMO1-polr3hMRPHLNO
      polr3kMO1-polr3kMRPHLNO
      tp53MO4-tp53MRPHLNO
      1 - 4 of 4
      Show
      Fish
      Antibodies
      Name Type Antigen Genes Isotypes Host Organism
      Ab1-crcppolyclonalRabbit
      Ab13-tp53polyclonal
        IgGRabbit
        1 - 2 of 2
        Show
        Orthology
        No data available
        Engineered Foreign Genes
        No data available
        Mapping
        No data available