PUBLICATION

Spinal motor neurons are regenerated after mechanical lesion and genetic ablation in larval zebrafish

Authors
Ohnmacht, J., Yang, Y.J., Maurer, G.W., Barreiro-Iglesias, A., Tsarouchas, T.M., Wehner, D., Sieger, D., Becker, C.G., Becker, T.
ID
ZDB-PUB-160312-9
Date
2016
Source
Development (Cambridge, England)   143(9): 1464-74 (Journal)
Registered Authors
Barreiro-Iglesias, Antón, Becker, Catherina G., Becker, Thomas, Ohnmacht, Jochen, Sieger, Dirk, Tsarouchas, Themistoklis, Wehner, Daniel, Yang, Yujie
Keywords
Dopamine, Macrophage, Microglia, Nitroreductase, Hb9, Olig2, Sox10
MeSH Terms
  • Motor Neurons/cytology*
  • Spinal Cord/cytology*
  • Neural Stem Cells/cytology*
  • Immunosuppressive Agents/pharmacology
  • Microglia/metabolism
  • PAX2 Transcription Factor/metabolism
  • Zebrafish/growth & development*
  • Nerve Regeneration/drug effects
  • Nerve Regeneration/physiology*
  • Zebrafish Proteins/metabolism
  • Larva/cytology*
  • Larva/genetics
  • Oligodendroglia/cytology
  • Animals
  • Dexamethasone/pharmacology
  • Immunity, Innate/drug effects
  • Metronidazole/pharmacology
  • Macrophages/immunology
  • Spinal Cord Injuries/metabolism*
(all 19)
PubMed
26965370 Full text @ Development
Abstract
In adult zebrafish, relatively quiescent progenitor cells show lesion-induced generation of motor neurons. Developmental motor neuron generation from the spinal motor neuron progenitor domain (pMN) sharply declines already at 48 hours post-fertilisation (hpf). After that, mostly oligodendrocytes are generated from the same domain. We demonstrate here that within 48 hours after a spinal lesion or specific genetic ablation of motor neurons at 72 hpf, the pMN domain reverts to motor neuron generation at the expense of oligodendrogenesis. In contrast, generation of dorsal Pax2-positive interneurons was not altered. Larval motor neuron regeneration can be boosted by dopaminergic drugs, similar to adult regeneration. We use larval lesions to show that pharmacological suppression of the cellular response of the innate immune system inhibits motor neuron regeneration. Hence, we have established a rapid larval regeneration paradigm. Both, mechanical lesion or motor neuron ablation are sufficient to reveal a high degree of developmental flexibility of pMN progenitor cells. In addition, we show an important influence of the immune system on motor neuron regeneration from these progenitor cells.
Genes / Markers
Figures
Figure Gallery (16 images) / 2
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Expression
Phenotype
No data available
Mutations / Transgenics
Allele Construct Type Affected Genomic Region
c264TgTransgenic Insertion
    cf3TgTransgenic Insertion
      e1TgTransgenic Insertion
        ml2TgTransgenic Insertion
          s300tTgTransgenic Insertion
            ue1TgTransgenic Insertion
              vu19TgTransgenic Insertion
                vu234TgTransgenic Insertion
                  1 - 8 of 8
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                  Human Disease / Model
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                  Sequence Targeting Reagents
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                  Fish
                  No data available
                  Antibodies
                  Orthology
                  No data available
                  Engineered Foreign Genes
                  Marker Marker Type Name
                  DsRed2EFGDsRed2
                  EGFPEFGEGFP
                  GAL4EFGGAL4
                  GFPEFGGFP
                  mCherryEFGmCherry
                  mRFPEFGmRFP
                  NTREFGNTR
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                  Mapping
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