PUBLICATION

Origin, specification and plasticity of the great vessels of the heart.

Authors

Nagelberg, D., Wang, J., Su, R., Torres-Vazquez, J., Targoff, K.L., Poss, K.D., Knaut, H.

ID

ZDB-PUB-151228-1

Date

2015

Source

Current biology : CB 25: 2099-2110 (Journal)

Registered Authors

Knaut, Holger, Nagelberg, Danielle, Poss, Kenneth D., Targoff, Kimara, Torres-Vázquez, Jesús, Wang, Jinhu

Keywords

none

MeSH Terms

Animals
Heart/embryology*
Branchial Region/embryology
Coronary Vessels/embryology
Transcription Factors/genetics
Transcription Factors/metabolism
Zebrafish/embryology*
Zebrafish/genetics
Fish Proteins/genetics
Fish Proteins/metabolism
Gene Expression Regulation, Developmental

(all 11)

PubMed

26255850 Full text @ Curr. Biol.

Abstract

The pharyngeal arch arteries (PAAs) are a series of paired embryonic blood vessels that give rise to several major arteries that connect directly to the heart. During development, the PAAs emerge from nkx2.5-expressing mesodermal cells and connect the dorsal head vasculature to the outflow tract of the heart. Despite their central role in establishing the circulatory system, the embryonic origins of the PAA progenitors are only coarsely defined, and the factors that specify them and their regenerative potential are unclear. Using fate mapping and mutant analysis, we find that PAA progenitors are derived from the tcf21 and nkx2.5 double-positive head mesoderm and require these two transcription factors for their specification and survival. Unexpectedly, cell ablation shows that the tcf21+; nkx2.5+ PAA progenitors are not required for PAA formation. We find that this compensation is due to the replacement of ablated tcf21+; nkx2.5+ PAA cells by endothelial cells from the dorsal head vasculature. Together, these studies assign the embryonic origin of the great vessel progenitors to the interface between the pharyngeal and cardiac mesoderm, identify the transcription factor code required for their specification, and reveal an unexpected plasticity in the formation of the great vessels.

Genes / Markers

Figures

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Expression

Gene	Fish	Conditions	Stage	Anatomy	Assay	Figure
DsRed2	fb7Tg; pd37Tg	standard conditions	Protruding-mouth	aortic arch 3	IFL	Fig. 4
DsRed2	fb7Tg; pd37Tg	standard conditions	Day 5	aortic arch 3	IFL	Fig. 4
DsRed2	fb7Tg; pd37Tg	standard conditions	Protruding-mouth	aortic arch 4	IFL	Fig. 4
DsRed2	fb7Tg; pd37Tg	standard conditions	Day 5	aortic arch 4	IFL	Fig. 4
DsRed2	fb7Tg; pd37Tg	standard conditions	Day 5	aortic arch 5	IFL	Fig. 4
DsRed2	fb7Tg; pd37Tg	standard conditions	Protruding-mouth	aortic arch 5	IFL	Fig. 4
DsRed2	fb7Tg; pd37Tg	standard conditions	Protruding-mouth	aortic arch 6	IFL	Fig. 4
DsRed2	fb7Tg; pd37Tg	standard conditions	Day 5	aortic arch 6	IFL	Fig. 4
DsRed2	fb7Tg; pd37Tg	standard conditions	Protruding-mouth	hypobranchial artery	IFL	Fig. 4
DsRed2	fb7Tg; pd37Tg	standard conditions	Day 5	hypobranchial artery	IFL	Fig. 4

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Phenotype

Fish	Conditions	Stage	Phenotype	Figure
fb7Tg; pd108Tg	chemical treatment: metronidazole	Protruding-mouth	head muscle mCherry expression absent, abnormal	Fig. 3
fb7Tg; pd108Tg	chemical treatment: metronidazole	Protruding-mouth	head muscle ZsYellow expression absent, abnormal	Fig. 3
fb7Tg; pd108Tg	chemical treatment: metronidazole	Protruding-mouth	pharyngeal musculature mCherry expression absent, abnormal	Fig. 3
fb7Tg; pd108Tg	chemical treatment: metronidazole	Protruding-mouth	pharyngeal musculature ZsYellow expression absent, abnormal	Fig. 3
fb7Tg; pd108Tg	chemical treatment: metronidazole	Protruding-mouth	pharyngeal vasculature mCherry expression absent, abnormal	Fig. 3
fb7Tg; pd108Tg	chemical treatment: metronidazole	Protruding-mouth	pharyngeal vasculature ZsYellow expression absent, abnormal	Fig. 3
fb7Tg; pd108Tg	chemical treatment: metronidazole	Day 5	head muscle mCherry expression absent, abnormal	Fig. 3
fb7Tg; pd108Tg	chemical treatment: metronidazole	Day 5	head muscle ZsYellow expression absent, abnormal	Fig. 3
fb7Tg; pd108Tg	chemical treatment: metronidazole	Day 5	pharyngeal musculature mCherry expression absent, abnormal	Fig. 3
fb7Tg; pd108Tg	chemical treatment: metronidazole	Day 5	pharyngeal musculature ZsYellow expression absent, abnormal	Fig. 3

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Mutations / Transgenics

Allele	Construct	Type
ba2Tg	Tg(-4.9sox10:EGFP)	Transgenic Insertion
fb7Tg	TgBAC(-36nkx2.5:ZsYellow)	Transgenic Insertion
hu4624Tg	TgBAC(flt1:Citrine)	Transgenic Insertion
la116Tg	Tg(kdrl:GFP)	Transgenic Insertion
mu101Tg	TgBAC(cdh5:GAL4FF)	Transgenic Insertion
pd37Tg	TgBAC(tcf21:DsRed2)	Transgenic Insertion
pd42Tg	TgBAC(cryaa:EGFP,tcf21:Cre-ERT2)	Transgenic Insertion
pd108Tg	TgBAC(tcf21:mCherry-NTR)	Transgenic Insertion
s896Tg	Tg(kdrl:Hsa.HRAS-mCherry)	Transgenic Insertion
s928Tg	Tg(-9.8actb2:LOXP-DsRed-LOXP-EGFP)	Transgenic Insertion

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Human Disease / Model

Sequence Targeting Reagents

Target	Reagent	Reagent Type
tcf21	TALEN2-tcf21	TALEN

Fish

Fish
ba2Tg; pd108Tg
fb7Tg; pd37Tg
fb7Tg; pd108Tg
fb7Tg; s896Tg
la116Tg
la116Tg; pd37Tg
la116Tg; pd108Tg
nkx2.5^vu179/vu179; fb7Tg; s896Tg
nkx2.5^vu179/vu179; tcf21^sk1/sk1
nkx2.5^vu179/vu179; tcf21^sk1/sk1; fb7Tg; s896Tg

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Antibodies

Orthology

Engineered Foreign Genes

Marker	Marker Type	Name
Cre	EFG	Cre
DsRed	EFG	DsRed
DsRed2	EFG	DsRed2
EGFP	EFG	EGFP
GAL4FF	EFG	GAL4FF
GFP	EFG	GFP
mCherry	EFG	mCherry
NTR	EFG	NTR
YFP	EFG	YFP
ZsYellow	EFG	ZsYellow

Mapping

Nagelberg et al., 2015 ZDB-PUB-151228-1 PMID:26255850

Origin, specification and plasticity of the great vessels of the heart.

Nagelberg et al., 2015

ZDB-PUB-151228-1
PMID:26255850