PUBLICATION

Müller glia provide essential tensile strength to the developing retina

Authors
MacDonald, R.B., Randlett, O., Oswald, J., Yoshimatsu, T., Franze, K., Harris, W.A.
ID
ZDB-PUB-150930-5
Date
2015
Source
The Journal of cell biology   210: 1075-83 (Journal)
Registered Authors
Harris, William A., MacDonald, Ryan, Randlett, Owen
Keywords
none
MeSH Terms
  • Ependymoglial Cells/metabolism*
  • Ependymoglial Cells/ultrastructure
  • Zebrafish/anatomy & histology
  • Zebrafish/embryology*
  • Retina/metabolism*
  • Retina/ultrastructure
  • Tensile Strength/physiology*
  • Animals
  • Microscopy, Atomic Force/methods
(all 9)
PubMed
26416961 Full text @ J. Cell Biol.
Abstract
To investigate the cellular basis of tissue integrity in a vertebrate central nervous system (CNS) tissue, we eliminated Müller glial cells (MG) from the zebrafish retina. For well over a century, glial cells have been ascribed a mechanical role in the support of neural tissues, yet this idea has not been specifically tested in vivo. We report here that retinas devoid of MG rip apart, a defect known as retinoschisis. Using atomic force microscopy, we show that retinas without MG have decreased resistance to tensile stress and are softer than controls. Laser ablation of MG processes showed that these cells are under tension in the tissue. Thus, we propose that MG act like springs that hold the neural retina together, finally confirming an active mechanical role of glial cells in the CNS.
Genes / Markers
Figures
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Expression
Phenotype
Mutations / Transgenics
Allele Construct Type Affected Genomic Region
cu2TgTransgenic Insertion
    jh16TgTransgenic Insertion
      kca6TgTransgenic Insertion
        mi6TgTransgenic Insertion
          nns5TgTransgenic Insertion
            nns17TgTransgenic Insertion
              q16bTgTransgenic Insertion
                q20TgTransgenic Insertion
                  s940TgTransgenic Insertion
                    tud106TgTransgenic Insertion
                      1 - 10 of 11
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                      Human Disease / Model
                      No data available
                      Sequence Targeting Reagents
                      Target Reagent Reagent Type
                      her4.1MO1-her4MRPHLNO
                      her4.3MO1-her4MRPHLNO
                      her4.4MO1-her4MRPHLNO
                      rbpjaMO1-rbpjaMRPHLNO
                      1 - 4 of 4
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                      Fish
                      Antibodies
                      Orthology
                      No data available
                      Engineered Foreign Genes
                      Marker Marker Type Name
                      CFPEFGCFP
                      CreEFGCre
                      EGFPEFGEGFP
                      GAL4EFGGAL4
                      GFPEFGGFP
                      mCherryEFGmCherry
                      RFPEFGRFP
                      TomatoEFGTomato
                      VenusEFGVenus
                      YFPEFGYFP
                      1 - 10 of 10
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                      Mapping
                      No data available