PUBLICATION

Spatiotemporal analysis of zebrafish hox gene regulation by Cdx4

Authors

Hayward, A.G., Joshi, P., Skromne, I.

ID

ZDB-PUB-150904-2

Date

2015

Source

Developmental Dynamics : an official publication of the American Association of Anatomists 244(12): 1564-73 (Journal)

Registered Authors

Skromne, Isaac

Keywords

Temporal collinearity, axial patterning, spatial collinearity

MeSH Terms

Body Patterning/genetics*
Transcriptional Activation
Zebrafish Proteins/genetics*
Zebrafish Proteins/metabolism
Homeodomain Proteins/genetics*
Homeodomain Proteins/metabolism
Animals
Genes, Homeobox/genetics*
Zebrafish/embryology*
Zebrafish/genetics
Gene Expression Regulation, Developmental*

(all 11)

PubMed

26335559 Full text @ Dev. Dyn.

Abstract

Background Cdx factors expressed in caudal regions of vertebrate embryos regulate hox patterning gene transcription. While loss of Cdx function is known to shift hox spatial expression domains posteriorly, the mechanism underlying the shift is not understood. We addressed this question by analyzing the spatiotemporal expression profile of all forty-nine zebrafish hox genes in wild type and Cdx4-deficient embryos.

Results Loss of Cdx4 had distinct effects on hox spatial expression in a paralogous group-dependent manner: in the head, group 4 expression was expanded posteriorly; in the trunk, group 5-10 expression was shifted posteriorly; and in the tail, group 11-13 genes were expressed in the tailbud but not in more differentiated tissues. In the trunk neural tissue, loss of Cdx4 severely delayed both transcriptional activation of hox genes during the initiation phase, and the anterior-ward expansion of hox expression domains during the establishment phase. In contrast, in the trunk mesoderm, loss of Cdx4 only delayed the hox initiation phase.

Conclusions These results indicate that Cdx4 differentially regulates the transcription of head, trunk and tail hox genes. In the trunk, Cdx4 conveys spatial positional information to axial tissues primarily by regulating the time of hox gene transcriptional activation during the initiation phase. This article is protected by copyright. All rights reserved.

Genes / Markers

Figures

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Expression

Gene	Fish	Conditions	Stage	Anatomy	Assay	Figure
egr2b	cdx4^{hi2188aTg/hi2188aTg}	standard conditions	20-25 somites	rhombomere 3	ISH	Fig. 2
egr2b	cdx4^{hi2188aTg/hi2188aTg}	standard conditions	20-25 somites	rhombomere 5	ISH	Fig. 2
egr2b	WT	standard conditions	20-25 somites	rhombomere 3	ISH	Fig. 2
egr2b	WT	standard conditions	20-25 somites	rhombomere 5	ISH	Fig. 2
hoxa9a	cdx4^{hi2188aTg/hi2188aTg}	standard conditions	20-25 somites	paraxial mesoderm anterior margin	ISH	Fig. 2
hoxa9a	cdx4^{hi2188aTg/hi2188aTg}	standard conditions	20-25 somites	spinal cord neural tube anterior margin	ISH	Fig. 2
hoxa9a	WT	standard conditions	20-25 somites	paraxial mesoderm	ISH	Fig. 2
hoxb4a	cdx4^{hi2188aTg/hi2188aTg}	standard conditions	20-25 somites	hindbrain posterior region	ISH	Fig. 2
hoxb4a	cdx4^{hi2188aTg/hi2188aTg}	standard conditions	20-25 somites	mesoderm posterior region	ISH	Fig. 2
hoxb4a	cdx4^{hi2188aTg/hi2188aTg}	standard conditions	20-25 somites	tail bud	ISH	Fig. 2

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Phenotype

Fish	Conditions	Stage	Phenotype	Figure
cdx4^{hi2188aTg/hi2188aTg}	standard conditions	20-25 somites	hindbrain posterior region hoxb4a expression increased distribution, abnormal	Fig. 2
cdx4^{hi2188aTg/hi2188aTg}	standard conditions	20-25 somites	hindbrain posterior region hoxb4a expression spatial pattern, abnormal	Fig. 2
cdx4^{hi2188aTg/hi2188aTg}	standard conditions	20-25 somites	mesoderm posterior region hoxb4a expression increased distribution, abnormal	Fig. 2
cdx4^{hi2188aTg/hi2188aTg}	standard conditions	20-25 somites	mesoderm posterior region hoxb4a expression spatial pattern, abnormal	Fig. 2
cdx4^{hi2188aTg/hi2188aTg}	standard conditions	20-25 somites	paraxial mesoderm hoxd4a expression decreased distribution, abnormal	Fig. 2
cdx4^{hi2188aTg/hi2188aTg}	standard conditions	20-25 somites	paraxial mesoderm hoxd4a expression spatial pattern, abnormal	Fig. 2
cdx4^{hi2188aTg/hi2188aTg}	standard conditions	20-25 somites	paraxial mesoderm anterior margin hoxa9a expression decreased distribution, abnormal	Fig. 2
cdx4^{hi2188aTg/hi2188aTg}	standard conditions	20-25 somites	paraxial mesoderm anterior margin hoxa9a expression spatial pattern, abnormal	Fig. 2
cdx4^{hi2188aTg/hi2188aTg}	standard conditions	20-25 somites	paraxial mesoderm anterior margin hoxb5a expression decreased distribution, abnormal	Fig. 2
cdx4^{hi2188aTg/hi2188aTg}	standard conditions	20-25 somites	paraxial mesoderm anterior margin hoxb5a expression spatial pattern, abnormal	Fig. 2

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Mutations / Transgenics

Allele	Construct	Type	Affected Genomic Region
hi2188aTg	Tg(nLacz-GTvirus)	Transgenic Insertion	cdx4
tv205c		Small Deletion	cdx4

Human Disease / Model

Sequence Targeting Reagents

Fish

Fish
cdx4^{hi2188aTg/hi2188aTg}
cdx4^{tv205c/tv205c}
WT

Antibodies

Name	Type	Antigen Genes	Isotypes	Host Organism
Ab-A4.1025	monoclonal		IgG2a	Mouse

Orthology

Engineered Foreign Genes

Mapping

Hayward et al., 2015 ZDB-PUB-150904-2 PMID:26335559

Spatiotemporal analysis of zebrafish hox gene regulation by Cdx4

Hayward et al., 2015

ZDB-PUB-150904-2
PMID:26335559