PUBLICATION

The transcriptional coactivator Taz regulates proximodistal patterning of the pronephric tubule in zebrafish

Authors

Zhang, J., Yuan, S., Vasilyev, A., Amin Arnaout, M.

ID

ZDB-PUB-150808-10

Date

2015

Source

Mechanisms of Development 138 Pt 3: 328-35 (Journal)

Registered Authors

Vasilyev, Aleksandr, Yuan, Shipeng, Zhang, Jiaojiao

Keywords

Taz, kidney cysts, patterning, pronephros, zebrafish

MeSH Terms

Kidney Tubules/cytology
Kidney Tubules/embryology*
Kidney Tubules/metabolism
Body Patterning/drug effects
Body Patterning/genetics
Body Patterning/physiology
Pronephros/cytology
Pronephros/embryology*
Pronephros/metabolism
Zebrafish Proteins/antagonists & inhibitors
Zebrafish Proteins/genetics
Zebrafish Proteins/physiology*
Zebrafish/embryology*
Zebrafish/genetics
Zebrafish/physiology*
Tretinoin/metabolism
Tretinoin/pharmacology
Gene Expression Regulation, Developmental
Gene Knockdown Techniques
Animals
Mesoderm/cytology
Mesoderm/embryology
Mesoderm/metabolism
Cell Count
Intracellular Signaling Peptides and Proteins/antagonists & inhibitors
Intracellular Signaling Peptides and Proteins/genetics
Intracellular Signaling Peptides and Proteins/physiology*
Animals, Genetically Modified

(all 28)

PubMed

26248207 Full text @ Mech. Dev.

Abstract

The zebrafish pronephric tubule consists of a proximal and distal segments and a collecting duct. The proximal segment is subdivided into the neck, proximal convoluted (PCT) and proximal straight (PST) tubule segments. The distal segment consists of a distal-early (DE) and late (DL) segments. How the proximal and distal segments develop along the anteroposterior axis is poorly understood. Here we show that knockdown of taz in zebrafish caused shortening and a significant reduction in number of principal cells of the PST-DE segment, and proximalization of the pronephric tubule in 24 hpf embryos. RA treatment expanded the pronephric proximal domain in normal embryos as in taz morphants, an effect that was further enhanced upon exposure of taz morphants to RA. The early pronephric defects in 24 hpf taz morphants led to failure of anterior pronephric tubule migration and convolution, and to PCT dilation and cyst formation in older embryos. In situ hybridization showed weak and transient expression of taz at the bud stage in intermediate mesoderm, the source of pronephric progenitors. The present findings show that Taz is required in anteroposterior patterning of the pronephric progenitor domain in intermediate mesoderm, acting in part by regulating RA signaling in pronephric progenitor field in intermediate mesoderm.

Genes / Markers

Figures

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Expression

Gene	Fish	Conditions	Stage	Anatomy	Assay	Figure
EGFP	la2Tg	control	Long-pec	pronephros multi-ciliated epithelial cell	IHC	Fig. 4
EGFP	la2Tg + MO1-wwtr1 + MO4-tp53	standard conditions	Long-pec	pronephros multi-ciliated epithelial cell	IHC	Fig. 4
EGFP	sqet11Et	control	Prim-5	pronephric distal early tubule	IHC	Fig. 2
EGFP	sqet11Et	control	Prim-5	pronephric distal early tubule	IHC	Fig. 3
EGFP	sqet11Et	control	Long-pec	pronephric distal early tubule	IHC	Fig. 3
EGFP	sqet11Et	control	Prim-5	pronephric proximal straight tubule	IHC	Fig. 3
EGFP	sqet11Et	control	Long-pec	pronephric proximal straight tubule	IHC	Fig. 3
EGFP	sqet11Et	control	Prim-5	pronephric proximal straight tubule	IHC	Fig. 2
EGFP	sqet11Et + MO1-wwtr1 + MO4-tp53	standard conditions	Prim-5	pronephric distal early tubule	IHC	Fig. 3
EGFP	sqet11Et + MO1-wwtr1 + MO4-tp53	standard conditions	Long-pec	pronephric distal early tubule	IHC	Fig. 3

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Phenotype

Fish	Conditions	Stage	Phenotype	Figure
WT + MO1-wwtr1 + MO4-tp53	standard conditions	Prim-5	pronephric proximal convoluted tubule position, normal	Fig. 1
WT + MO1-wwtr1 + MO4-tp53	standard conditions	Long-pec	pronephric proximal convoluted tubule mislocalised posteriorly, abnormal	Fig. 1
WT + MO1-wwtr1 + MO4-tp53	standard conditions	Long-pec	pronephric proximal tubule development process quality, abnormal	Fig. 1
WT + MO1-wwtr1 + MO4-tp53	standard conditions	Long-pec	pronephric tubule orientation whole organism anterior-posterior axis, abnormal	Fig. 1
WT + MO1-wwtr1 + MO4-tp53	standard conditions	Protruding-mouth	pronephric proximal convoluted tubule straight, abnormal	Fig. 1
WT + MO1-wwtr1 + MO4-tp53	standard conditions	Protruding-mouth	pronephric proximal tubule development process quality, abnormal	Fig. 1
la2Tg + MO1-wwtr1 + MO4-tp53	standard conditions	Long-pec	pronephric duct multi-ciliated epithelial cell amount, normal	Fig. 4
la2Tg + MO1-wwtr1 + MO4-tp53	standard conditions	Long-pec	pronephric duct multi-ciliated epithelial cell functionality, normal	Fig. 4
sqet11Et	chemical treatment: 4-(diethylamino)benzaldehyde	Prim-5	pronephric distal early tubule decreased length, abnormal	Fig. 5
sqet11Et	chemical treatment: 4-(diethylamino)benzaldehyde	Prim-5	pronephric distal late tubule increased length, abnormal	Fig. 5

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Mutations / Transgenics

Allele	Construct	Type	Affected Genomic Region
la2Tg	Tg(-6.0itga2b:EGFP)	Transgenic Insertion
sqet11Et	Et(krt4:EGFP)	Transgenic Insertion

Human Disease / Model

Sequence Targeting Reagents

Target	Reagent	Reagent Type
tp53	MO4-tp53	MRPHLNO
wwtr1	MO1-wwtr1	MRPHLNO

Fish

Fish
la2Tg
la2Tg + MO1-wwtr1 + MO4-tp53
sqet11Et
sqet11Et + MO1-wwtr1 + MO4-tp53
WT
WT + MO1-wwtr1 + MO4-tp53

Antibodies

Name	Type	Antigen Genes	Isotypes	Host Organism
Ab1-atp1a1	monoclonal		IgG2a	Mouse
Ab-MF20	monoclonal		IgG2b	Mouse

Orthology

Engineered Foreign Genes

Marker	Marker Type	Name
EGFP	EFG	EGFP

Mapping

Zhang et al., 2015 ZDB-PUB-150808-10 PMID:26248207

The transcriptional coactivator Taz regulates proximodistal patterning of the pronephric tubule in zebrafish

Zhang et al., 2015

ZDB-PUB-150808-10
PMID:26248207