PUBLICATION

Homeobox transcription factor Six7 governs expression of green opsin genes in zebrafish

Authors
Ogawa, Y., Shiraki, T., Kojima, D., Fukada, Y.
ID
ZDB-PUB-150717-6
Date
2015
Source
Proceedings. Biological sciences   282(1812): 20150659 (Journal)
Registered Authors
Fukada, Yoshitaka, Kojima, Daisuke, Ogawa, Yohey
Keywords
cone opsin, green opsin, photoreceptor development, six7, zebrafish (Danio rerio)
MeSH Terms
  • Homeodomain Proteins/genetics*
  • Homeodomain Proteins/metabolism
  • Rod Opsins/genetics*
  • Rod Opsins/metabolism
  • Zebrafish Proteins/genetics*
  • Zebrafish Proteins/metabolism
  • Zebrafish/genetics*
  • Zebrafish/growth & development
  • Zebrafish/metabolism
  • Gene Expression Regulation, Developmental*
  • Real-Time Polymerase Chain Reaction
  • Retinal Cone Photoreceptor Cells/metabolism*
  • Animals
  • In Situ Hybridization
(all 14)
PubMed
26180064 Full text @ Proc. Biol. Sci.
Abstract
Colour discrimination in vertebrates requires cone photoreceptor cells in the retina, and high-acuity colour vision is endowed by a set of four cone subtypes expressing UV-, blue-, green- and red-sensitive opsins. Previous studies identified transcription factors governing cone photoreceptor development in mice, although loss of blue and green opsin genes in the evolution of mammals make it difficult to understand how high-acuity colour vision was organized during evolution and development. Zebrafish (Danio rerio) represents a valuable vertebrate model for studying colour vision as it retains all the four ancestral vertebrate cone subtypes. Here, by RT-qPCR and in situ hybridization analysis, we found that sine oculis homeobox homolog 7 (six7), a transcription factor widely conserved in ray-finned fish, is expressed predominantly in the cone photoreceptors in zebrafish at both the larval and the adult stages. TAL effector nuclease-based six7 knock-out revealed its roles in expression of green, red and blue cone opsin genes. Most prominently, the six7 deficiency caused a loss of expression of all the green opsins at both the larval and adult stages. six7 is indispensable for the development and/or maintenance of the green cones.
Genes / Markers
Figures
No images available
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Expression
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay Figure
arr3azpr-1EKWstandard conditionsProtruding-mouthIHC
arr3azpr-1six7ja51/ja51standard conditionsProtruding-mouthIHC
gnat1ja23Tgstandard conditionsAdultRTPCR
gnat1ja2Tgstandard conditionsAdultRTPCR
gnat2ja23Tgstandard conditionsAdultRTPCR
gnat2ja2Tgstandard conditionsAdultNot DetectedRTPCR
opn1lw1EKWstandard conditionsAdultISH
opn1lw1six7ja51/ja51standard conditionsAdultISH
opn1lw2EKWstandard conditionsAdultISH
opn1lw2six7ja51/ja51standard conditionsAdultISH
1 - 10 of 40
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Phenotype
Fish Conditions Stage Phenotype Figure
six3bja53/ja53; six7ja52/ja52standard conditionsLong-pec
six7ja51/ja51standard conditionsProtruding-mouth
six7ja51/ja51standard conditionsProtruding-mouth
six7ja51/ja51standard conditionsAdult
six7ja51/ja51standard conditionsAdult
1 - 5 of 5
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Mutations / Transgenics
Allele Construct Type Affected Genomic Region
ja2TgTransgenic Insertion
    ja23TgTransgenic Insertion
      ja51
        		Indel
        ja52
          		Small Deletion
          ja53
            		Small Deletion
            ja54
              		Indel
              ja55
                		Small Deletion
                1 - 7 of 7
                Show
                Human Disease / Model
                No data available
                Sequence Targeting Reagents
                Target Reagent Reagent Type
                six3bTALEN1-six3bTALEN
                six7TALEN1-six7TALEN
                1 - 2 of 2
                Show
                Fish
                Fish
                EKW
                ja2Tg
                ja23Tg
                six3bja53/ja53; six7ja52/ja52
                six7ja51/ja51
                1 - 5 of 5
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                Antibodies
                Orthology
                Engineered Foreign Genes
                Marker Marker Type Name
                EGFPEFGEGFP
                1 - 1 of 1
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                Mapping
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                Citation
                Ogawa, Y., Shiraki, T., Kojima, D., Fukada, Y. (2015) Homeobox transcription factor Six7 governs expression of green opsin genes in zebrafish. Proceedings. Biological sciences. 282(1812):20150659.