PUBLICATION

Identification of polarized macrophage subsets in zebrafish

Authors
Nguyen Chi, M., Laplace-Builhe, B., Travnickova, J., Luz-Crawford, P., Tejedor, G., Phan, Q.T., Duroux-Richard, I., Levraud, J.P., Kissa, K., Lutfalla, G., Jorgensen, C., Djouad, F.
ID
ZDB-PUB-150711-11
Date
2015
Source
eLIFE   4: e07288 (Journal)
Registered Authors
Djouad, Farida, Kissa-Marin, Karima, Levraud, Jean-Pierre, Lutfalla, Georges, Phan, Quang Tien
Keywords
developmental biology, immunology, live imaging, macrophages, stem cells, zebrafish
MeSH Terms
  • Escherichia coli Infections/immunology
  • Real-Time Polymerase Chain Reaction
  • Sequence Analysis, DNA
  • Animals
  • Flow Cytometry
  • Gene Expression Profiling
  • Tumor Necrosis Factor-alpha/biosynthesis
  • Genes, Reporter
  • Microscopy, Confocal
  • Molecular Sequence Data
  • Macrophages/classification*
  • Macrophages/immunology*
  • Reverse Transcriptase Polymerase Chain Reaction
  • Animals, Genetically Modified
  • Wounds and Injuries/immunology
  • Zebrafish/immunology*
(all 16)
PubMed
26154973 Full text @ Elife
Abstract
While the mammalian macrophage phenotypes have been intensively studied in vitro, the dynamic of their phenotypic polarization has never been investigated in live vertebrates. We used the zebrafish as a live model to identify and trail macrophage subtypes. We generated a transgenic line whose macrophages expressing tnfa, a key feature of classically activated (M1) macrophages, express fluorescent proteins Tg(mpeg1:mCherryF/tnfa:eGFP-F). Using 4D-confocal microscopy, we showed that both aseptic wounding and E. coli inoculation triggered macrophage recruitment, some of which started to express tnfa. RT-qPCR on FACS-sorted tnfa+ and tnfa- macrophages showed that they respectively expressed M1 and alternatively activated (M2) mammalian markers. Fate tracing of tnfa+ macrophages during the time-course of inflammation demonstrated that pro-inflammatory macrophages converted into M2-like phenotype during the resolution step. Our results reveal the diversity and plasticity of zebrafish macrophage subsets and underline the similarities with mammalian macrophages proposing a new system to study macrophage functional dynamic.
Genes / Markers
Figures
Figure Gallery (5 images)
Show all Figures
Expression
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay Figure
EGFPump2Tg; ump5Tgphysical alteration: anatomical structureProtruding-mouthIFL
EGFPump2Tg; ump5Tgphysical alteration: anatomical structureProtruding-mouthIFL
EGFPump2Tg; ump5Tgphysical alteration: anatomical structureProtruding-mouthIFL
EGFPump2Tg; ump5TgcontrolProtruding-mouthNot DetectedIFL
EGFPump5TgcontrolProtruding-mouthIFL
EGFPump5Tgphysical alteration: anatomical structureProtruding-mouthIFL
EGFPump5TgcontrolProtruding-mouthNot DetectedIFL
EGFPump5Tgbacterial treatmentProtruding-mouthIFL
Kaedegl25Tg; rk8Tgphysical alteration: anatomical structureProtruding-mouth to Day 4IFL
mCherryump2Tg; ump5Tgphysical alteration: anatomical structureProtruding-mouthIFL
1 - 10 of 17
Show
Phenotype
No data available
Mutations / Transgenics
Allele Construct Type Affected Genomic Region
gl25TgTransgenic Insertion
    rk8TgTransgenic Insertion
      ump2TgTransgenic Insertion
        ump5TgTransgenic Insertion
          1 - 4 of 4
          Show
          Human Disease / Model
          No data available
          Sequence Targeting Reagents
          No data available
          Fish
          Fish
          AB
          gl25Tg; rk8Tg
          ump2Tg; ump5Tg
          ump5Tg
          1 - 4 of 4
          Show
          Antibodies
          No data available
          Orthology
          No data available
          Engineered Foreign Genes
          Marker Marker Type Name
          EGFPEFGEGFP
          GAL4FFEFGGAL4FF
          KaedeEFGKaede
          mCherryEFGmCherry
          1 - 4 of 4
          Show
          Mapping
          No data available
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          Citation
          Nguyen Chi, M., Laplace-Builhe, B., Travnickova, J., Luz-Crawford, P., Tejedor, G., Phan, Q.T., Duroux-Richard, I., Levraud, J.P., Kissa, K., Lutfalla, G., Jorgensen, C., Djouad, F. (2015) Identification of polarized macrophage subsets in zebrafish. eLIFE. 4:e07288.