PUBLICATION

Neurodevelopment. Live imaging of adult neural stem cell behavior in the intact and injured zebrafish brain

Authors
Barbosa, J.S., Sanchez-Gonzalez, R., Di Giaimo, R., Baumgart, E.V., Theis, F.J., Götz, M., Ninkovic, J.
ID
ZDB-PUB-150516-3
Date
2015
Source
Science (New York, N.Y.)   348: 789-93 (Journal)
Registered Authors
Ninkovic, Jovica
Keywords
none
MeSH Terms
  • Neural Stem Cells/cytology*
  • Adult Stem Cells/cytology*
  • Neurogenesis*
  • Brain/cytology*
  • Brain/physiology*
  • Neuroimaging
  • Brain Injuries/pathology
  • Brain Injuries/physiopathology
  • Regeneration*
  • Animals
  • Cell Division
  • Telencephalon/cytology
  • Telencephalon/injuries
  • Telencephalon/physiology
  • Neurons/cytology*
  • Zebrafish/physiology*
(all 16)
PubMed
25977550 Full text @ Science
Abstract
Adult neural stem cells are the source for restoring injured brain tissue. We used repetitive imaging to follow single stem cells in the intact and injured adult zebrafish telencephalon in vivo and found that neurons are generated by both direct conversions of stem cells into postmitotic neurons and via intermediate progenitors amplifying the neuronal output. We observed an imbalance of direct conversion consuming the stem cells and asymmetric and symmetric self-renewing divisions, leading to depletion of stem cells over time. After brain injury, neuronal progenitors are recruited to the injury site. These progenitors are generated by symmetric divisions that deplete the pool of stem cells, a mode of neurogenesis absent in the intact telencephalon. Our analysis revealed changes in the behavior of stem cells underlying generation of additional neurons during regeneration.
Genes / Markers
Figures
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Expression
No data available
Phenotype
No data available
Mutations / Transgenics
Allele Construct Type Affected Genomic Region
knu3TgTransgenic Insertion
    mi2001TgTransgenic Insertion
      1 - 2 of 2
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      Human Disease / Model
      No data available
      Sequence Targeting Reagents
      No data available
      Fish
      No data available
      Antibodies
      Orthology
      No data available
      Engineered Foreign Genes
      Marker Marker Type Name
      EGFPEFGEGFP
      GFPEFGGFP
      1 - 2 of 2
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      Mapping
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      Citation
      Barbosa, J.S., Sanchez-Gonzalez, R., Di Giaimo, R., Baumgart, E.V., Theis, F.J., Götz, M., Ninkovic, J. (2015) Neurodevelopment. Live imaging of adult neural stem cell behavior in the intact and injured zebrafish brain. Science (New York, N.Y.). 348:789-93.