PUBLICATION

Tight junction protein 1a regulates pigment cell organisation during zebrafish colour patterning

Authors

Fadeev, A., Krauss, J., Frohnhöfer, H.G., Irion, U., Nüsslein-Volhard, C.

ID

ZDB-PUB-150429-6

Date

2015

Source

eLIFE 4: (Journal)

Registered Authors

Fadeev, Andrey, Frohnhöfer, Hans Georg, Irion, Uwe, Krauss, Jana, Nüsslein-Volhard, Christiane

Keywords

ZO-1, cell biology, chromatophores, colour pattern formation, developmental biology, iridophores, stem cells, tight junction protein, zebrafish

MeSH Terms

Animals
Zebrafish Proteins/metabolism*
Cell Movement/physiology
Zonula Occludens-1 Protein/metabolism*
Pigmentation/physiology
Metamorphosis, Biological/physiology*
Zebrafish
Body Patterning/physiology*

(all 8)

PubMed

25915619 Full text @ Elife

Abstract

Zebrafish display a prominent pattern of alternating dark and light stripes generated by the precise positioning of pigment cells in the skin. This arrangement is the result of coordinated cell movements, cell shape changes and the organisation of pigment cells during metamorphosis. Iridophores play a crucial part in this process by switching between the dense form of the light stripes and the loose form of the dark stripes. Adult schachbrett (sbr) mutants exhibit delayed changes in iridophore shape and organisation caused by truncations in Tight Junction Protein 1a (ZO-1a). In sbr mutants, the dark stripes are interrupted by dense iridophores invading as coherent sheets. Immuno-labelling and chimeric analyses indicate that Tjp1a is expressed in dense iridophores, but down-regulated in the loose form. Tjp-1a is a novel regulator of cell shape changes during colour pattern formation and the first cytoplasmic protein implicated in this process.

Genes / Markers

Figures

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Expression

Gene	Fish	Conditions	Stage	Anatomy	Assay	Figure
EGFP	s843Tg	standard conditions	Adult	blood vessel	IHC	Fig. 7, S2
EGFP	s843Tg	standard conditions	Day 5	blood vessel	IHC	Fig. 7, S2
GFP	t32238Tg; tdl358Et	standard conditions	Days 14-20 to Days 21-29	iridophore	IFL	Fig. 4
GFP	tdl358Et	standard conditions	Days 21-29	iridophore	IHC	Fig. 7
GFP	tdl358Et	standard conditions	Days 14-20 to Days 30-44	iridophore	IFL	Fig. 3
GFP	tdl358Et	standard conditions	Days 14-20	iridophore	IHC	Fig. 7
GFP	tjp1a^{tnh009b/tnh009b}; t32238Tg; tdl358Et	standard conditions	Days 14-20 to Days 21-29	iridophore	IFL	Fig. 4
GFP	tjp1a^{tnh009b/tnh009b}; tdl358Et	standard conditions	Days 14-20 to Days 30-44	iridophore	IFL	Fig. 3
mCherry	tjp1a^{tnh009b/tnh009b}; hzm1Et	standard conditions	Days 21-29	melanocyte	IFL	Fig. 5
RFP	t32238Tg; tdl358Et	standard conditions	Days 14-20 to Days 21-29	pigment cell membrane	IFL	Fig. 4

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Phenotype

Fish	Conditions	Stage	Phenotype	Figure
csf1ra^{tm236b/tm236b}	standard conditions	Adult	xanthophore absent, abnormal	Fig. 6
csf1ra^{tm236b/tm236b}; tjp1a^{tnh009b/tnh009b}	standard conditions	Adult	iridophore pigmentation process quality, abnormal	Fig. 6
csf1ra^{tm236b/tm236b}; tjp1a^{tnh009b/tnh009b}	standard conditions	Adult	pigmentation process quality, abnormal	Fig. 6
gja4^{t32241/t32241}	standard conditions	Adult	iridophore pigmentation process quality, abnormal	Fig. 8
gja4^{t32241/t32241}	standard conditions	Adult	melanophore stripe broken, abnormal	Fig. 8
gja4^{t32241/t32241}	standard conditions	Adult	pigmentation process quality, abnormal	Fig. 8
gja5b^t1/t1	standard conditions	Adult	iridophore pigmentation process quality, abnormal	Fig. 8
gja5b^t1/t1	standard conditions	Adult	melanophore stripe broken, abnormal	Fig. 8
gja5b^t1/t1	standard conditions	Adult	pigmentation process quality, abnormal	Fig. 8
gja5b^t1/t1; tjp1a^{tnh009b/tnh009b}	standard conditions	Adult	iridophore pigmentation process quality, abnormal	Fig. 8

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Mutations / Transgenics

Allele	Construct	Type	Affected Genomic Region
b134		Unknown	kita
hzm1Et	Et(kita:GAL4TA4,UAS:mCherry)	Transgenic Insertion
j9s1		Point Mutation	ltk
s843Tg	Tg(kdrl:EGFP)	Transgenic Insertion
t1		Point Mutation	gja5b
t32238Tg	Tg2(sox10:mRFP)	Transgenic Insertion
t32241		Indel	gja4
t32243Tg	Tg(h2az2a:GFP)	Transgenic Insertion
tdl358Et	Et(Ola.Edar:GAL4,14xUAS:GFP)	Transgenic Insertion
tm236b		Point Mutation	csf1ra

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Human Disease / Model

Sequence Targeting Reagents

Target	Reagent	Reagent Type
tjp1b	MO1-tjp1b	MRPHLNO

Fish

Fish
csf1ra^{tm236b/tm236b}
csf1ra^{tm236b/tm236b}; tjp1a^{tnh009b/tnh009b}
gja4^{t32241/t32241}
gja5b^t1/t1
gja5b^t1/t1; tjp1a^{tnh009b/tnh009b}
kita^b134/b134
ltk^j9s1/j9s1
ltk^j9s1/j9s1; tjp1a^{tnh009b/tnh009b}
mitfa^w2/w2
mitfa^w2/w2; tjp1a^{tnh009b/tnh009b}

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Antibodies

Name	Type	Antigen Genes	Isotypes	Host Organism
Ab2-cdh1	monoclonal		IgG2a	Mouse
Ab4-tjp1a	polyclonal	tjp1a		Rabbit
Ab5-tjp1a	polyclonal	tjp1a		Rabbit

Orthology

Engineered Foreign Genes

Marker	Marker Type	Name
EGFP	EFG	EGFP
GAL4	EFG	GAL4
GAL4TA4	EFG	GAL4TA4
GFP	EFG	GFP
mCherry	EFG	mCherry
mRFP	EFG	mRFP
RFP	EFG	RFP

Mapping

Fadeev et al., 2015 ZDB-PUB-150429-6 PMID:25915619

Tight junction protein 1a regulates pigment cell organisation during zebrafish colour patterning

Fadeev et al., 2015

ZDB-PUB-150429-6
PMID:25915619