PUBLICATION

Robust regeneration of adult zebrafish lateral line hair cells reflects continued precursor pool maintenance

Authors
Cruz, I.A., Kappedal, R., Mackenzie, S.M., Hailey, D.W., Hoffman, T.L., Schilling, T.F., Raible, D.W.
ID
ZDB-PUB-150415-6
Date
2015
Source
Developmental Biology   402(2): 229-38 (Journal)
Registered Authors
Raible, David, Schilling, Tom
Keywords
Adult zebrafish, hair cells, lateral line, neuromasts, regeneration
MeSH Terms
  • Lateral Line System/cytology*
  • Lateral Line System/physiology*
  • Zebrafish/physiology*
  • Regeneration/physiology*
  • Fluorescence
  • Immunohistochemistry
  • Animals
  • Neomycin
  • Bromodeoxyuridine
  • Mechanoreceptors/physiology*
(all 10)
PubMed
25869855 Full text @ Dev. Biol.
Abstract
We have examined lateral line hair cell and support cell maintenance in adult zebrafish when growth is largely complete. We demonstrate that adult zebrafish not only replenish hair cells after a single instance of hair cell damage, but also maintain hair cells and support cells after multiple rounds of damage and regeneration. We find that hair cells undergo continuous turnover in adult zebrafish in the absence of damage. We identify mitotically-distinct support cell populations and show that hair cells regenerate from underlying support cells in a region-specific manner. Our results demonstrate that there are two distinct support cell populations in the lateral line, which may help explain why zebrafish hair cell regeneration is extremely robust, retained throughout life, and potentially unlimited in regenerative capacity.
Genes / Markers
Figures
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Expression
Phenotype
No data available
Mutations / Transgenics
Allele Construct Type Affected Genomic Region
s356tTgTransgenic Insertion
    sqet20EtTransgenic Insertion
      w100TgTransgenic Insertion
        w191TgTransgenic Insertion
          zf704TgTransgenic Insertion
            1 - 5 of 5
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            Human Disease / Model
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            Sequence Targeting Reagents
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            Fish
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            Antibodies
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            Orthology
            No data available
            Engineered Foreign Genes
            Marker Marker Type Name
            EGFPEFGEGFP
            EosEFGEos
            GFPEFGGFP
            TomatoEFGTomato
            1 - 4 of 4
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            Mapping
            No data available
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            Citation
            Cruz, I.A., Kappedal, R., Mackenzie, S.M., Hailey, D.W., Hoffman, T.L., Schilling, T.F., Raible, D.W. (2015) Robust regeneration of adult zebrafish lateral line hair cells reflects continued precursor pool maintenance. Developmental Biology. 402(2):229-38.