PUBLICATION

Eph-Pak2a signaling regulates branching of the pharyngeal endoderm by inhibiting late-stage epithelial dynamics

Authors
Choe, C.P., Crump, J.G.
ID
ZDB-PUB-150301-5
Date
2015
Source
Development (Cambridge, England)   142(6): 1089-94 (Journal)
Registered Authors
Crump, Gage DeKoeyer
Keywords
none
MeSH Terms
  • Morpholinos/genetics
  • Receptor, EphB4/metabolism*
  • In Situ Hybridization, Fluorescence
  • Zebrafish Proteins/metabolism*
  • Branchial Region/embryology*
  • Epithelial Cells/physiology*
  • Morphogenesis/physiology*
  • Microscopy, Confocal
  • Time-Lapse Imaging
  • Animals
  • Zebrafish/embryology*
  • Signal Transduction/genetics
  • Signal Transduction/physiology*
  • Immunohistochemistry
  • Protein Serine-Threonine Kinases/metabolism*
(all 15)
PubMed
25725065 Full text @ Development
Abstract
Branching morphogenesis depends on the precise temporal and spatial control of epithelial dynamics. In the vertebrate head, endodermal branches, called pharyngeal pouches, form through the transient stratification, collective migration and reorganization of epithelial cells into bilayers. Here, we report novel requirements for the EphrinB ligands B2a and B3b, the Ephb4a receptor and the Pak2a kinase in the development of pouches and the posterior facial skeleton that depends on pouches for its segmentation. Time-lapse imaging in zebrafish shows that EphB-Pak2a signaling is required to stabilize pouch epithelial cells at the end of branching morphogenesis. Transgenic rescue experiments further demonstrate that endodermal Eph-ephrin signaling promotes pouch integrity by targeting Pak2a to the plasma membrane, where subsequent activation by Wnt4a-Cdc42 signaling increases junctional E-cadherin in maturing pouches. Integration of Eph-ephrin and Wnt4a signaling through Pak2a thus signals the end of branching morphogenesis by increasing intercellular adhesion that blocks further epithelial rearrangements.
Genes / Markers
Figures
Figure Gallery (9 images)
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Expression
Phenotype
No data available
Mutations / Transgenics
Allele Construct Type Affected Genomic Region
el8TgTransgenic Insertion
    el72TgTransgenic Insertion
      el93TgTransgenic Insertion
        el225TgTransgenic Insertion
          el268TgTransgenic Insertion
            el321TgTransgenic Insertion
              el530TgTransgenic Insertion
                el589TgTransgenic Insertion
                  el590TgTransgenic Insertion
                    el605TgTransgenic Insertion
                      1 - 10 of 15
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                      Human Disease / Model
                      No data available
                      Sequence Targeting Reagents
                      Target Reagent Reagent Type
                      efnb3bMO2-efnb3bMRPHLNO
                      efnb3bMO3-efnb3bMRPHLNO
                      1 - 2 of 2
                      Show
                      Fish
                      Fish
                      efnb2ahu3393/hu3393
                      el8Tg
                      el72Tg
                      el93Tg
                      el225Tg
                      el268Tg
                      el321Tg
                      el530Tg
                      el589Tg
                      el605Tg
                      1 - 10 of 12
                      Show
                      Antibodies
                      Orthology
                      No data available
                      Engineered Foreign Genes
                      Marker Marker Type Name
                      CeruleanEFGCerulean
                      EGFPEFGEGFP
                      GAL4EFGGAL4
                      GFPEFGGFP
                      mCherryEFGmCherry
                      1 - 5 of 5
                      Show
                      Mapping
                      No data available
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                      Citation
                      Choe, C.P., Crump, J.G. (2015) Eph-Pak2a signaling regulates branching of the pharyngeal endoderm by inhibiting late-stage epithelial dynamics. Development (Cambridge, England). 142(6):1089-94.