PUBLICATION

Defective apical extrusion signaling contributes to aggressive tumor hallmarks

Authors
Gu, Y., Shea, J., Slattum, G., Firpo, M.A., Alexander, M., Mulvihill, S.J., Golubovskaya, V.M., Rosenblatt, J.
ID
ZDB-PUB-150127-2
Date
2015
Source
eLIFE   4: e04069 (Journal)
Registered Authors
Keywords
carcinoma, cell biology, epithelial, extrusion, human, human biology, invasion, medicine, mouse, pancreatic, tumor initiation, zebrafish
MeSH Terms
  • Pancreatic Neoplasms/metabolism*
  • Pancreatic Neoplasms/pathology*
  • Animals
  • Cell Aggregation/drug effects
  • Focal Adhesion Protein-Tyrosine Kinases/antagonists & inhibitors
  • Focal Adhesion Protein-Tyrosine Kinases/metabolism
  • Apoptosis/drug effects
  • Madin Darby Canine Kidney Cells
  • Models, Biological
  • Signal Transduction*/drug effects
  • Receptors, Lysosphingolipid/metabolism
  • Epithelial Cells/drug effects
  • Epithelial Cells/pathology
  • Cell Polarity*/drug effects
  • Neoplasm Metastasis
  • Carcinoma, Pancreatic Ductal/metabolism
  • Carcinoma, Pancreatic Ductal/pathology
  • Zebrafish/embryology
  • Neoplasm Invasiveness
  • Dogs
  • Embryo, Nonmammalian/drug effects
  • Embryo, Nonmammalian/pathology
  • Protein Kinase Inhibitors/pharmacology
  • Humans
  • Cell Line, Tumor
  • Epidermis/drug effects
  • Epidermis/embryology
  • Epidermis/pathology
(all 28)
PubMed
25621765 Full text @ Elife
Abstract
When epithelia become too crowded, some cells are extruded that later die. To extrude, a cell produces the lipid, Sphingosine 1-Phosphate (S1P), which activates S1P2 receptors in neighboring cells that seamlessly squeeze the cell out of the epithelium. Here, we find that extrusion defects can contribute to carcinogenesis and tumor progression. Tumors or epithelia lacking S1P2 cannot extrude cells apically and instead form apoptotic-resistant masses, possess poor barrier function, and shift extrusion basally beneath the epithelium, providing a potential mechanism for cell invasion. Exogenous S1P2 expression is sufficient to rescue apical extrusion, cell death, and reduce orthotopic pancreatic tumors and their metastases. Focal Adhesion Kinase (FAK) inhibitor can bypass extrusion defects and could, therefore, target pancreatic, lung, and colon tumors that lack S1P2 without affecting wild-type tissue.
Genes / Markers
Figures
Figure Gallery (2 images)
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Expression
No data available
Phenotype
Fish Conditions Stage Phenotype Figure
WT + MO1-s1pr2controlDay 5
WT + MO1-s1pr2chemical treatment: EC 2.7.10.2 (non-specific protein-tyrosine kinase) inhibitorDay 5
s1pr2m93/m93standard conditionsDay 5
s1pr2m93/m93chemical treatment: EC 2.7.10.2 (non-specific protein-tyrosine kinase) inhibitorDay 5
1 - 4 of 4
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Mutations / Transgenics
Allele Construct Type Affected Genomic Region
m93
    		Point Mutation
    1 - 1 of 1
    Show
    Human Disease / Model
    Human Disease Fish Conditions Evidence
    carcinomas1pr2m93/m93standard conditionsTAS
    1 - 1 of 1
    Show
    Sequence Targeting Reagents
    Target Reagent Reagent Type
    s1pr2MO1-s1pr2MRPHLNO
    1 - 1 of 1
    Show
    Fish
    Fish
    s1pr2m93/m93
    WT + MO1-s1pr2
    1 - 2 of 2
    Show
    Antibodies
    No data available
    Orthology
    No data available
    Engineered Foreign Genes
    No data available
    Mapping
    No data available
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    Citation
    Gu, Y., Shea, J., Slattum, G., Firpo, M.A., Alexander, M., Mulvihill, S.J., Golubovskaya, V.M., Rosenblatt, J. (2015) Defective apical extrusion signaling contributes to aggressive tumor hallmarks. eLIFE. 4:e04069.