PUBLICATION

The adhesion GPCR Gpr56 regulates oligodendrocyte development via interactions with Gα12/13 and RhoA

Authors
Ackerman, S.D., Garcia, C., Piao, X., Gutmann, D.H., Monk, K.R.
ID
ZDB-PUB-150122-4
Date
2015
Source
Nature communications   6: 6122 (Journal)
Registered Authors
Monk, Kelly
Keywords
Biological sciences, Neuroscience, Developmental biology
MeSH Terms
  • Cell Differentiation
  • Cell Proliferation
  • Mice, Knockout
  • Mice
  • Amino Acid Sequence
  • Zebrafish
  • Myelin Sheath/metabolism
  • Neurons/metabolism
  • Zebrafish Proteins/metabolism*
  • Gene Expression Regulation, Developmental*
  • Monomeric GTP-Binding Proteins/metabolism*
  • Molecular Sequence Data
  • Sequence Homology, Amino Acid
  • rho GTP-Binding Proteins/metabolism*
  • Cell Adhesion
  • Oligodendroglia/metabolism*
  • Axons/metabolism
  • Female
  • Animals
  • Mutation
  • Microscopy, Electron, Transmission
  • Receptors, G-Protein-Coupled/metabolism
  • Receptors, G-Protein-Coupled/physiology*
  • Male
  • Mice, Inbred C57BL
  • GTP-Binding Protein alpha Subunits, G12-G13/metabolism*
(all 26)
PubMed
25607772 Full text @ Nat. Commun.
Abstract
In the vertebrate central nervous system, myelinating oligodendrocytes are postmitotic and derive from proliferative oligodendrocyte precursor cells (OPCs). The molecular mechanisms that govern oligodendrocyte development are incompletely understood, but recent studies implicate the adhesion class of G protein-coupled receptors (aGPCRs) as important regulators of myelination. Here, we use zebrafish and mouse models to dissect the function of the aGPCR Gpr56 in oligodendrocyte development. We show that gpr56 is expressed during early stages of oligodendrocyte development. In addition, we observe a significant reduction of mature oligodendrocyte number and myelinated axons in gpr56 zebrafish mutants. This reduction results from decreased OPC proliferation, rather than increased cell death or altered neural precursor differentiation potential. Finally, we show that these functions are mediated by Gα12/13 proteins and Rho activation. Together, our data establish Gpr56 as a regulator of oligodendrocyte development.
Genes / Markers
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Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping
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Citation
Ackerman, S.D., Garcia, C., Piao, X., Gutmann, D.H., Monk, K.R. (2015) The adhesion GPCR Gpr56 regulates oligodendrocyte development via interactions with Gα12/13 and RhoA. Nature communications. 6:6122.