PUBLICATION

Interkinetic Nuclear Migration Is Centrosome Independent and Ensures Apical Cell Division to Maintain Tissue Integrity

Authors
Strzyz, P.J., Lee, H.O., Sidhaye, J., Weber, I.P., Leung, L.C., Norden, C.
ID
ZDB-PUB-150121-8
Date
2015
Source
Developmental Cell   32(2): 203-19 (Journal)
Registered Authors
Norden, Caren, Sidhaye, Jaydeep, Strzyz, Paulina, Weber, Isabell
Keywords
none
MeSH Terms
  • Cell Nucleus/metabolism*
  • Cell Nucleus/pathology
  • Zebrafish/metabolism*
  • Cell Division/physiology*
  • Epithelial Cells/cytology*
  • Centrosome/metabolism*
  • Dietary Sucrose/metabolism
  • Epithelium/metabolism
  • Epithelium/pathology
  • Animals
(all 10)
PubMed
25600237 Full text @ Dev. Cell
Abstract
Pseudostratified epithelia are widespread during animal development and feature elongated cells whose nuclei adopt various positions along the apicobasal cell axis. Before mitosis, nuclei migrate toward the apical surface, and subsequent divisions occur apically. So far, the exact purpose of this nuclear migration remained elusive. One hypothesis was that apical migration ensures that nuclei and centrosomes meet for mitosis. We here demonstrate that in zebrafish neuroepithelia apical nuclear migration occurs independently of centrosome position or integrity. It is a highly reproducible phenomenon linked to the cell cycle via CDK1 activity. We propose that the robustness of bringing nuclei apically for mitosis ensures that cells are capable of reintegrating into the epithelium after division. Nonapical divisions lead to cell delamination and formation of cell clusters that subsequently interfere with neuronal layering. Therefore, positioning divisions apically in pseudostratified neuroepithelia could serve to safeguard epithelial integrity and enable proper proliferation and maturation.
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rw021TgTransgenic Insertion
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    Sequence Targeting Reagents
    Target Reagent Reagent Type
    prkciMO1-prkciMRPHLNO
    prkczMO1-prkczMRPHLNO
    tp53MO4-tp53MRPHLNO
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    Marker Marker Type Name
    GFPEFGGFP
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    Citation
    Strzyz, P.J., Lee, H.O., Sidhaye, J., Weber, I.P., Leung, L.C., Norden, C. (2015) Interkinetic Nuclear Migration Is Centrosome Independent and Ensures Apical Cell Division to Maintain Tissue Integrity. Developmental Cell. 32(2):203-19.