PUBLICATION

Spatial pattern of cell geometry and cell-division orientation in zebrafish lens epithelium

Authors
Mochizuki, T., Suzuki, S., Masai, I.
ID
ZDB-PUB-140928-4
Date
2014
Source
Biology Open   3(10): 982-94 (Journal)
Registered Authors
Masai, Ichiro, Mochizuki, Toshiaki
Keywords
E-cadherin, cell division, cell proliferation, lens, zebrafish
MeSH Terms
none
PubMed
25260917 Full text @ Biol. Open
Abstract
Cell proliferation is a key regulator of tissue morphogenesis. We examined cell proliferation and cell division in zebrafish lens epithelium by visualizing cell-cycle phases and nuclear positions, using fluorescent-labeled geminin and histone proteins. Proliferation was low in the anterior region of lens epithelium and higher in the marginal zone anterior to the equator, suggesting that the proliferation zone, called the germinative zone, is formed in zebrafish lens. Interestingly, cell-division orientation was biased longitudinally in the anterior region, shifted from longitudinal to circumferential along the anterior-posterior axis of lens sphere, and was biased circumferentially in the peripheral region. These data suggest that cell-division orientation is spatially regulated in zebrafish lens epithelium. The Hertwig rule indicates that cells tend to divide along their long axes. Orientation of long axes and cell division were biased similarly in zebrafish lens epithelium, suggesting that cell geometry correlates with cell-division orientation. A cell adhesion molecule, E-cadherin, is expressed in lens epithelium. In a zebrafish e-cadherin mutant, the long axes and cell-division orientation were shifted more longitudinally. These data suggest that E-cadherin is required for the spatial pattern of cell geometry and cell-division orientation in zebrafish lens epithelium.
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Mutations / Transgenics
Allele Construct Type Affected Genomic Region
kca6TgTransgenic Insertion
    kca66TgTransgenic Insertion
      oki011TgTransgenic Insertion
        rk3
          		Point Mutation
          1 - 4 of 4
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          Fish
          cdh1rk3/rk3
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          Engineered Foreign Genes
          Marker Marker Type Name
          GFPEFGGFP
          mCherryEFGmCherry
          1 - 2 of 2
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          Citation
          Mochizuki, T., Suzuki, S., Masai, I. (2014) Spatial pattern of cell geometry and cell-division orientation in zebrafish lens epithelium. Biology Open. 3(10):982-94.