PUBLICATION

Smoc2 modulates embryonic myelopoiesis during zebrafish development

Authors
Mommaerts, H., Esguerra, C.V., Hartmann, U., Luyten, F.P., Tylzanowski, P.
ID
ZDB-PUB-140722-8
Date
2014
Source
Developmental Dynamics : an official publication of the American Association of Anatomists   243(11): 1375-90 (Journal)
Registered Authors
Esguerra, Camila V.
Keywords
Alk8, Bmp, Sparc, Spi1b, hematopoiesis
MeSH Terms
  • Mesoderm/metabolism
  • Zebrafish/embryology*
  • Embryo, Nonmammalian/drug effects
  • Calcium-Binding Proteins/metabolism*
  • Gene Expression Regulation, Developmental/genetics
  • Gene Expression Regulation, Developmental/physiology*
  • Myelopoiesis/drug effects
  • Myelopoiesis/genetics*
  • Myelopoiesis/physiology*
  • Hematopoiesis/physiology
  • Extracellular Matrix Proteins/metabolism*
  • Zebrafish Proteins/metabolism*
  • Animals
(all 13)
PubMed
25044883 Full text @ Dev. Dyn.
Abstract
Background: SMOC2 is a member of the BM-40 (SPARC) family of matricellular proteins, reported to influence signaling in the extracellular compartment. In mice, Smoc2 is expressed in many different tissues and was shown to enhance the response to angiogenic growth factors, mediate cell adhesion, keratinocyte migration and metastasis. Additionally, SMOC2 is associated with vitiligo and craniofacial and dental defects. The function of Smoc2 during early zebrafish development has not been determined to date. Results: In pregastrula zebrafish embryos, smoc2 is expressed ubiquitously. As development progresses, the expression pattern becomes more anteriorly restricted. At the onset of blood cell circulation, smoc2 morphants presented a mild ventralization of posterior structures. Molecular analysis of the smoc2 morphants indicated myelopoietic defects in the rostral blood islands during segmentation stages. Hemangioblast development and further specification of the myeloid progenitor cells were shown to be impaired. Additional experiments indicated that Bmp target genes were downregulated in smoc2 morphants. Conclusion: Our findings reveal that Smoc2 is an essential player in the development of myeloid cells of the anterior lateral plate mesoderm during embryonic zebrafish development. Furthermore, our data show that Smoc2 affects the transcription of Bmp target genes without affecting initial dorsoventral patterning or mesoderm development.
Genes / Markers
Figures
Figure Gallery (10 images)
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Expression
Phenotype
Mutations / Transgenics
Allele Construct Type Affected Genomic Region
i114TgTransgenic Insertion
    twu34TgTransgenic Insertion
      y1TgTransgenic Insertion
        1 - 3 of 3
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        Human Disease / Model
        No data available
        Sequence Targeting Reagents
        Target Reagent Reagent Type
        smoc2MO5-smoc2MRPHLNO
        smoc2MO6-smoc2MRPHLNO
        tp53MO4-tp53MRPHLNO
        1 - 3 of 3
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        Fish
        Antibodies
        No data available
        Orthology
        No data available
        Engineered Foreign Genes
        Marker Marker Type Name
        EGFPEFGEGFP
        GFPEFGGFP
        1 - 2 of 2
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        Mapping
        No data available