PUBLICATION

Distinct regulation of the anterior and posterior myeloperoxidase expression by Etv2 and Gata1 during primitive Granulopoiesis in zebrafish

Authors
Glenn, N.O., Schumacher, J.A., Kim, H.J., Zhao, E.J., Skerniskyte, J., Sumanas, S.
ID
ZDB-PUB-140624-1
Date
2014
Source
Developmental Biology   393(1): 149-59 (Journal)
Registered Authors
Glenn, Nikki, Kim, Hyon, Schumacher, Jennifer, Sumanas, Saulius
Keywords
Er71, Etsrp, Etv2, Gata1, Granulocytes, Myeloid, Neutrophil, Primitive hematopoiesis, Zebrafish
MeSH Terms
  • Morpholinos/genetics
  • Gene Expression Regulation, Developmental
  • Zebrafish/blood
  • Zebrafish/embryology*
  • Troponin T/genetics
  • Embryo, Nonmammalian
  • Basic Helix-Loop-Helix Transcription Factors/genetics
  • Gene Knockdown Techniques
  • Peroxidase/biosynthesis*
  • Peroxidase/genetics
  • Leukopoiesis*
  • Mesoderm/embryology
  • Mesoderm/metabolism
  • Neutrophils/cytology*
  • Trans-Activators/biosynthesis
  • Trans-Activators/genetics
  • GATA1 Transcription Factor/biosynthesis
  • GATA1 Transcription Factor/genetics*
  • Hematopoietic Stem Cells/cytology
  • Hematopoietic Stem Cells/physiology
  • Animals
  • Zebrafish Proteins/biosynthesis
  • Zebrafish Proteins/genetics*
  • Proto-Oncogene Proteins/biosynthesis
  • Proto-Oncogene Proteins/genetics
(all 25)
PubMed
24956419 Full text @ Dev. Biol.
Abstract
Neutrophilic granulocytes are the most abundant type of myeloid cells and form an essential part of the innate immune system. In vertebrates the first neutrophils are thought to originate during primitive hematopoiesis, which precedes hematopoietic stem cell formation. In zebrafish embryos, it has been suggested that primitive neutrophils may originate in two distinct sites, the anterior (ALPM) and posterior lateral plate mesoderm (PLPM). An ETS-family transcription factor Etsrp/Etv2/ER71 has been implicated in vasculogenesis and hematopoiesis in multiple vertebrates. However, its role during neutrophil development is not well understood. Here we demonstrate using zebrafish embryos that Etv2 has a specific cell-autonomous function during primitive neutropoiesis in the anterior lateral plate mesoderm (ALPM) but has little effect on erythropoiesis or the posterior lateral plate mesoderm (PLPM) expression of neutrophil marker myeloperoxidase mpo/mpx. Our results argue that ALPM-derived neutrophils originate from etv2-expressing cells which downregulate etv2 during neutropoiesis. We further show that Scl functions downstream of Etv2 in anterior neutropoiesis. Additionally, we demonstrate that mpx expression within the PLPM overlaps with gata1 expression, potentially marking the cells with a dual myelo-erythroid potential. Intriguingly, initiation of mpx expression in the PLPM is dependent on gata1 but not etv2 function. Our results demonstrate that mpx expression is controlled differently in the ALPM and PLPM regions and describe novel roles for etv2 and gata1 during primitive neutropoiesis.
Genes / Markers
Figures
Figure Gallery (8 images)
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Expression
Phenotype
No data available
Mutations / Transgenics
Allele Construct Type Affected Genomic Region
ci1TgTransgenic Insertion
    ci6TgTransgenic Insertion
      uwm1TgTransgenic Insertion
        y1TgTransgenic Insertion
          y11
            Insertion
            1 - 5 of 5
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            Human Disease / Model
            No data available
            Sequence Targeting Reagents
            Target Reagent Reagent Type
            etsrpMO1-etsrpMRPHLNO
            etsrpMO2-etsrpMRPHLNO
            gata1aMO1-gata1aMRPHLNO
            spi1bMO2-spi1bMRPHLNO
            spi1bMO3-spi1bMRPHLNO
            tal1MO4-tal1MRPHLNO
            tnnt2aMO1-tnnt2aMRPHLNO
            1 - 7 of 7
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            Fish
            Antibodies
            No data available
            Orthology
            No data available
            Engineered Foreign Genes
            Marker Marker Type Name
            EGFPEFGEGFP
            GFPEFGGFP
            KaedeEFGKaede
            1 - 3 of 3
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            Mapping
            No data available