PUBLICATION

The Evolution and Appearance of C3 Duplications in Fish Originate an Exclusive Teleost c3 Gene Form with Anti-Inflammatory Activity

Authors
Forn-Cuní, G., Reis, E.S., Dios, S., Posada, D., Lambris, J.D., Figueras, A., Novoa, B.
ID
ZDB-PUB-140614-2
Date
2014
Source
PLoS One   9: e99673 (Journal)
Registered Authors
Figueras, Antonio, Novoa, Beatriz
Keywords
Zebrafish, Morpholino, Neutrophils, Gene expression, Genomic databases, Inflammation, Phylogenetic analysis, Larvae
MeSH Terms
  • Cytokines/metabolism
  • Complement C3/genetics*
  • Complement C3/immunology
  • Gene Duplication*
  • Zebrafish/genetics*
  • Zebrafish/immunology
  • Zebrafish/microbiology
  • Multigene Family
  • Zebrafish Proteins/genetics*
  • Zebrafish Proteins/immunology
  • Aeromonas hydrophila/immunology
  • Animals
  • Evolution, Molecular
(all 13)
PubMed
24926798 Full text @ PLoS One
Abstract
The complement system acts as a first line of defense and promotes organism homeostasis by modulating the fates of diverse physiological processes. Multiple copies of component genes have been previously identified in fish, suggesting a key role for this system in aquatic organisms. Herein, we confirm the presence of three different previously reported complement c3 genes (c3.1, c3.2, c3.3) and identify five additional c3 genes (c3.4, c3.5, c3.6, c3.7, c3.8) in the zebrafish genome. Additionally, we evaluate the mRNA expression levels of the different c3 genes during ontogeny and in different tissues under steady-state and inflammatory conditions. Furthermore, while reconciling the phylogenetic tree with the fish species tree, we uncovered an event of c3 duplication common to all teleost fishes that gave rise to an exclusive c3 paralog (c3.7 and c3.8). These paralogs showed a distinct ability to regulate neutrophil migration in response to injury compared with the other c3 genes and may play a role in maintaining the balance between inflammatory and homeostatic processes in zebrafish.
Genes / Markers
Figures
Figure Gallery (4 images)
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Expression
Phenotype
Mutations / Transgenics
Allele Construct Type Affected Genomic Region
i114TgTransgenic Insertion
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    Human Disease / Model
    No data available
    Sequence Targeting Reagents
    Target Reagent Reagent Type
    c3a.1MO1-c3aMRPHLNO
    c3a.1MO1-c3a.1MRPHLNO
    c3a.2MO1-c3aMRPHLNO
    c3a.3MO1-c3aMRPHLNO
    c3a.6MO1-c3aMRPHLNO
    c3b.1MO1-c3bMRPHLNO
    c3b.1MO2-c3bMRPHLNO
    c3b.2MO1-c3bMRPHLNO
    c3b.2MO2-c3bMRPHLNO
    1 - 9 of 9
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    Fish
    Antibodies
    No data available
    Orthology
    1 - 8 of 8
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    Engineered Foreign Genes
    Marker Marker Type Name
    GFPEFGGFP
    1 - 1 of 1
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    Mapping
    No data available