PUBLICATION

Vegfd can compensate for loss of Vegfc in zebrafish facial lymphatic sprouting

Authors
Astin, J.W., Haggerty, M.J., Okuda, K.S., Le Guen, L., Misa, J.P., Tromp, A., Hogan, B.M., Crosier, K.E., Crosier, P.S.
ID
ZDB-PUB-140607-9
Date
2014
Source
Development (Cambridge, England)   141(13): 2680-90 (Journal)
Registered Authors
Crosier, Phil, Hogan, Ben M., Le Guen, Ludovic, Okuda, Kazuhide Shaun
Keywords
none
MeSH Terms
  • Reverse Transcriptase Polymerase Chain Reaction
  • Microscopy, Confocal
  • Lymphangiogenesis/genetics
  • Lymphangiogenesis/physiology*
  • DNA Primers/genetics
  • Statistics, Nonparametric
  • Vascular Endothelial Growth Factor D/metabolism*
  • Zebrafish Proteins/deficiency*
  • Zebrafish Proteins/metabolism*
  • Vascular Endothelial Growth Factor C/deficiency*
  • In Situ Hybridization
  • Calcium-Binding Proteins/metabolism
  • Morpholinos/genetics
  • Animals
  • Zebrafish/embryology*
(all 15)
PubMed
24903752 Full text @ Development
Abstract
Lymphangiogenesis is a dynamic process that involves the sprouting of lymphatic endothelial cells (LECs) from veins to form lymphatic vessels. Vegfr3 signalling, through its ligand Vegfc and the extracellular protein Ccbe1, is essential for the sprouting of LECs to form the trunk lymphatic network. In this study we determined whether Vegfr3, Vegfc and Ccbe1 are also required for development of the facial and intestinal lymphatic networks in the zebrafish embryo. Whereas Vegfr3 and Ccbe1 are required for the development of all lymphatic vessels, Vegfc is dispensable for facial lymphatic sprouting but not for the complete development of the facial lymphatic network. We show that zebrafish vegfd is expressed in the head, genetically interacts with ccbe1 and can rescue the lymphatic defects observed following the loss of vegfc. Finally, whereas knockdown of vegfd has no phenotype, double knockdown of both vegfc and vegfd is required to prevent facial lymphatic sprouting, suggesting that Vegfc is not essential for all lymphatic sprouting and that Vegfd can compensate for loss of Vegfc during lymphatic development in the zebrafish head.
Genes / Markers
Figures
Figure Gallery (13 images) / 2
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Expression
Phenotype
Fish Conditions Stage Phenotype Figure
flt4hu4602/hu4602; nz150Tgstandard conditionsProtruding-mouth
flt4hu4602/hu4602; nz150Tgstandard conditionsDay 5
flt4hu4602/hu4602; nz150Tgstandard conditionsDay 5
nz101Tg; s843Tg + MO1-ccbe1standard conditionsDay 6
nz101Tg; s843Tg + MO3-flt4standard conditionsDay 6
nz101Tg; s843Tg + MO3-vegfcstandard conditionsDay 6
nz150Tg + MO1-ccbe1standard conditionsPrim-25
nz150Tg + MO1-ccbe1standard conditionsProtruding-mouth
nz150Tg + MO1-ccbe1standard conditionsDay 5
nz150Tg + MO1-ccbe1standard conditionsDay 5
1 - 10 of 40
Show
Mutations / Transgenics
Allele Construct Type Affected Genomic Region
hu4602
    		Point Mutation
    hu5055
      		Point Mutation
      la4TgTransgenic Insertion
        nz101TgTransgenic Insertion
          nz150TgTransgenic Insertion
            s843TgTransgenic Insertion
              1 - 6 of 6
              Show
              Human Disease / Model
              No data available
              Sequence Targeting Reagents
              Target Reagent Reagent Type
              ccbe1MO1-ccbe1MRPHLNO
              cxcl12aMO6-cxcl12aMRPHLNO
              cxcr4aMO8-cxcr4aMRPHLNO
              flt4MO3-flt4MRPHLNO
              vegfcMO3-vegfcMRPHLNO
              vegfcMO4-vegfcMRPHLNO
              vegfdMO1-vegfdMRPHLNO
              1 - 7 of 7
              Show
              Fish
              Fish
              flt4hu4602/hu4602
              flt4hu4602/hu4602; nz150Tg
              la4Tg
              la4Tg; nz150Tg
              nz101Tg
              nz101Tg; s843Tg
              nz101Tg; s843Tg + MO1-ccbe1
              nz101Tg; s843Tg + MO1-vegfd
              nz101Tg; s843Tg + MO3-flt4
              nz101Tg; s843Tg + MO3-vegfc
              1 - 10 of 28
              Show
              Antibodies
              No data available
              Orthology
              No data available
              Engineered Foreign Genes
              Marker Marker Type Name
              DsRedEFGDsRed
              EGFPEFGEGFP
              RFPEFGRFP
              1 - 3 of 3
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              Mapping
              No data available
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              Citation
              Astin, J.W., Haggerty, M.J., Okuda, K.S., Le Guen, L., Misa, J.P., Tromp, A., Hogan, B.M., Crosier, K.E., Crosier, P.S. (2014) Vegfd can compensate for loss of Vegfc in zebrafish facial lymphatic sprouting. Development (Cambridge, England). 141(13):2680-90.