PUBLICATION

A Pak1/Erk Signaling Module Acts through Gata6 to Regulate Cardiovascular Development in Zebrafish

Authors
Kelly, M.L., Astsaturov, A., Rhodes, J., Chernoff, J.
ID
ZDB-PUB-140516-11
Date
2014
Source
Developmental Cell   29: 350-9 (Journal)
Registered Authors
Rhodes, Jennifer
Keywords
none
MeSH Terms
  • GATA Transcription Factors/metabolism*
  • p21-Activated Kinases/genetics
  • p21-Activated Kinases/metabolism*
  • Zebrafish Proteins/metabolism*
  • MAP Kinase Kinase 1/metabolism
  • Morpholinos/genetics
  • Extracellular Signal-Regulated MAP Kinases/metabolism*
  • Cardiovascular System/embryology*
  • Neural Crest/embryology*
  • Gene Knockdown Techniques
  • Cardiovascular Abnormalities/genetics
  • Phosphorylation
  • Signal Transduction/genetics
  • Cell Movement
  • Heart/embryology
  • Zebrafish/embryology*
  • Zebrafish/genetics
  • Zebrafish/metabolism
  • Animals
  • Gene Expression Regulation, Developmental
(all 20)
PubMed
24823378 Full text @ Dev. Cell
Abstract
Proper neural crest development and migration is critical during embryonic development, but the molecular mechanisms regulating this process remain incompletely understood. Here, we show that the protein kinase Erk, which plays a central role in a number of key developmental processes in vertebrates, is regulated in the developing neural crest by p21-activated protein kinase 1 (Pak1). Furthermore, we show that activated Erk signals by phosphorylating the transcription factor Gata6 on a conserved serine residue to promote neural crest migration and proper formation of craniofacial structures, pigment cells, and the outflow tract of the heart. Our data suggest an essential role for Pak1 as an Erk activator, and Gata6 as an Erk target, during neural crest development.
Genes / Markers
Figures
Figure Gallery (7 images)
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Expression
Phenotype
Mutations / Transgenics
Allele Construct Type Affected Genomic Region
sd2TgTransgenic Insertion
    twu34TgTransgenic Insertion
      1 - 2 of 2
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      Human Disease / Model
      No data available
      Sequence Targeting Reagents
      Target Reagent Reagent Type
      pak1MO2-pak1MRPHLNO
      pak2aMO1-pak2aMRPHLNO
      pak2bMO1-pak2bMRPHLNO
      tp53MO4-tp53MRPHLNO
      1 - 4 of 4
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      Fish
      Antibodies
      Orthology
      No data available
      Engineered Foreign Genes
      Marker Marker Type Name
      DsRedEFGDsRed
      EGFPEFGEGFP
      1 - 2 of 2
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      Mapping
      No data available