PUBLICATION

Cadm4 Restricts the Production of Cardiac Outflow Tract Progenitor Cells

Authors
Zeng, X.X., Yelon, D.
ID
ZDB-PUB-140513-6
Date
2014
Source
Cell Reports   7(4): 951-60 (Journal)
Registered Authors
Yelon, Deborah, Zeng, Sean
Keywords
none
Datasets
GEO:GSE55540
MeSH Terms
  • Cell Differentiation/physiology
  • Zebrafish Proteins/genetics
  • Zebrafish Proteins/metabolism
  • Zebrafish Proteins/physiology*
  • Zebrafish
  • Animals, Genetically Modified
  • Signal Transduction
  • Cell Adhesion Molecules/genetics
  • Cell Adhesion Molecules/metabolism
  • Cell Adhesion Molecules/physiology*
  • Cell Growth Processes/physiology
  • Gene Knockdown Techniques
  • Cardiac Output/physiology*
  • Stem Cells/cytology*
  • Stem Cells/metabolism
  • Myocardium/cytology*
  • Myocardium/metabolism
  • Animals
(all 18)
PubMed
24813897 Full text @ Cell Rep.
Abstract
Heart assembly requires input from two populations of progenitor cells, the first and second heart fields (FHF and SHF), that differentiate at distinct times and create different cardiac components. The cardiac outflow tract (OFT) is built through recruitment of late-differentiating, SHF-derived cardiomyocytes to the arterial pole of the heart. The mechanisms responsible for selection of an appropriate number of OFT cells from the SHF remain unclear. Here, we find that cell adhesion molecule 4 (cadm4) is essential for restricting the size of the zebrafish OFT. Knockdown of cadm4 causes dramatic OFT expansion, and overexpression of cadm4 results in a greatly diminished OFT. Moreover, cadm4 activity limits the production of OFT progenitor cells and the duration of their accumulation at the arterial pole. Together, our data suggest a role for cell adhesion in restraining SHF deployment to the OFT, perturbation of which could cause congenital OFT defects.
Genes / Markers
Figures
Figure Gallery (8 images)
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Expression
Phenotype
Mutations / Transgenics
Allele Construct Type Affected Genomic Region
f2TgTransgenic Insertion
    fb7TgTransgenic Insertion
      fb9TgTransgenic Insertion
        pd3TgTransgenic Insertion
          sd22TgTransgenic Insertion
            sk74TgTransgenic Insertion
              ti282a
                Point Mutation
                twu277TgTransgenic Insertion
                  1 - 8 of 8
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                  Human Disease / Model
                  No data available
                  Sequence Targeting Reagents
                  Target Reagent Reagent Type
                  cadm4MO1-cadm4MRPHLNO
                  cadm4MO2-cadm4MRPHLNO
                  cadm4MO3-cadm4MRPHLNO
                  1 - 3 of 3
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                  Fish
                  Antibodies
                  Name Type Antigen Genes Isotypes Host Organism
                  Ab-MF20monoclonal
                    IgG2bMouse
                    Ab-S46monoclonalIgG1Mouse
                    1 - 2 of 2
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                    Orthology
                    No data available
                    Engineered Foreign Genes
                    Marker Marker Type Name
                    DsRedEFGDsRed
                    DsRed2EFGDsRed2
                    EGFPEFGEGFP
                    KaedeEFGKaede
                    ZsYellowEFGZsYellow
                    1 - 5 of 5
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                    Mapping
                    No data available