PUBLICATION

Wnt-regulated dynamics of positional information in zebrafish somitogenesis

Authors
Bajard, L., Morelli, L.G., Ares, S., Pécréaux, J., Jülicher, F., Oates, A.C.
ID
ZDB-PUB-140513-413
Date
2014
Source
Development (Cambridge, England)   141: 1381-91 (Journal)
Registered Authors
Oates, Andrew
Keywords
Embryonic elongation, Fgf signaling, Segmentation clock, Signal gradient, Time-lapse microscopy, Wnt signaling
MeSH Terms
  • Models, Biological
  • Body Patterning/genetics
  • Body Patterning/physiology
  • Wnt Proteins/antagonists & inhibitors
  • Wnt Proteins/genetics
  • Wnt Proteins/physiology
  • Wnt Signaling Pathway/genetics
  • Wnt Signaling Pathway/physiology*
  • Somites/embryology*
  • Somites/metabolism*
  • Basic Helix-Loop-Helix Transcription Factors/genetics
  • Basic Helix-Loop-Helix Transcription Factors/physiology
  • Heat-Shock Response/genetics
  • Heat-Shock Response/physiology
  • Zebrafish/embryology*
  • Zebrafish/genetics
  • Zebrafish/metabolism*
  • Gene Expression Regulation, Developmental
  • Zebrafish Proteins/antagonists & inhibitors
  • Zebrafish Proteins/genetics
  • Zebrafish Proteins/physiology*
  • Intercellular Signaling Peptides and Proteins/genetics
  • Intercellular Signaling Peptides and Proteins/physiology
  • Animals
  • Animals, Genetically Modified
  • Fibroblast Growth Factors/genetics
  • Fibroblast Growth Factors/physiology
  • T-Box Domain Proteins/genetics
  • T-Box Domain Proteins/physiology
(all 29)
PubMed
24595291 Full text @ Development
Abstract
How signaling gradients supply positional information in a field of moving cells is an unsolved question in patterning and morphogenesis. Here, we ask how a Wnt signaling gradient regulates the dynamics of a wavefront of cellular change in a flow of cells during somitogenesis. Using time-controlled perturbations of Wnt signaling in the zebrafish embryo, we changed segment length without altering the rate of somite formation or embryonic elongation. This result implies specific Wnt regulation of the wavefront velocity. The observed Wnt signaling gradient dynamics and timing of downstream events support a model for wavefront regulation in which cell flow plays a dominant role in transporting positional information.
Genes / Markers
Figures
Figure Gallery (9 images)
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Expression
Phenotype
Fish Conditions Stage Phenotype Figure
w32Tg (AB)heat shock5-9 somites to Prim-5
w34Tg (AB)heat shock5-9 somites to Prim-5
w35Tg (AB)heat shock5-9 somites to Prim-5
1 - 3 of 3
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Mutations / Transgenics
Allele Construct Type Affected Genomic Region
w32TgTransgenic Insertion
    w34TgTransgenic Insertion
      w35TgTransgenic Insertion
        1 - 3 of 3
        Show
        Human Disease / Model
        No data available
        Sequence Targeting Reagents
        No data available
        Fish
        Fish
        w32Tg (AB)
        w34Tg (AB)
        w35Tg (AB)
        WT
        1 - 4 of 4
        Show
        Antibodies
        Orthology
        Engineered Foreign Genes
        Marker Marker Type Name
        GFPEFGGFP
        YFPEFGYFP
        1 - 2 of 2
        Show
        Mapping
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        Citation
        Bajard, L., Morelli, L.G., Ares, S., Pécréaux, J., Jülicher, F., Oates, A.C. (2014) Wnt-regulated dynamics of positional information in zebrafish somitogenesis. Development (Cambridge, England). 141:1381-91.