PUBLICATION

Distinct Notch signaling outputs pattern the developing arterial system

Authors
Quillien, A., Moore, J.C., Shin, M., Siekmann, A.F., Smith, T., Pan, L., Moens, C.B., Parsons, M.J., Lawson, N.D.
ID
ZDB-PUB-140513-406
Date
2014
Source
Development (Cambridge, England)   141: 1544-52 (Journal)
Registered Authors
Lawson, Nathan, Moens, Cecilia, Moore, John, Pan, Luyuan, Parsons, Michael, Shin, Masahiro, Siekmann, Arndt Friedrich, Smith, Tom
Keywords
Artery differentiation, Notch, Vascular system, Zebrafish
MeSH Terms
  • Zebrafish/embryology
  • Zebrafish/genetics
  • Neovascularization, Physiologic/genetics
  • Veins/embryology
  • Receptors, Notch/physiology*
  • Arteries/cytology
  • Arteries/embryology*
  • Animals
  • Animals, Genetically Modified
  • Morphogenesis/genetics
  • Endothelium, Vascular/embryology
  • Signal Transduction/physiology
  • Body Patterning/genetics*
  • Cell Differentiation/genetics
  • Embryo, Nonmammalian
(all 15)
PubMed
24598161 Full text @ Development
Abstract
Differentiation of arteries and veins is essential for the development of a functional circulatory system. In vertebrate embryos, genetic manipulation of Notch signaling has demonstrated the importance of this pathway in driving artery endothelial cell differentiation. However, when and where Notch activation occurs to affect endothelial cell fate is less clear. Using transgenic zebrafish bearing a Notch-responsive reporter, we demonstrate that Notch is activated in endothelial progenitors during vasculogenesis prior to blood vessel morphogenesis and is maintained in arterial endothelial cells throughout larval stages. Furthermore, we find that endothelial progenitors in which Notch is activated are committed to a dorsal aorta fate. Interestingly, some arterial endothelial cells subsequently downregulate Notch signaling and then contribute to veins during vascular remodeling. Lineage analysis, together with perturbation of both Notch receptor and ligand function, further suggests several distinct developmental windows in which Notch signaling acts to promote artery commitment and maintenance. Together, these findings demonstrate that Notch acts in distinct contexts to initiate and maintain artery identity during embryogenesis.
Genes / Markers
Figures
Figure Gallery (10 images)
Show all Figures
Expression
Phenotype
Fish Conditions Stage Phenotype Figure
WT + MO6-notch1bstandard conditionsProtruding-mouth
dll4j16e1/j16e1standard conditionsProtruding-mouth
1 - 2 of 2
Show
Mutations / Transgenics
Allele Construct Type Affected Genomic Region
fh332
    		Point Mutation
    j16e1
      		Point Mutation
      jh12TgTransgenic Insertion
        jh15TgTransgenic Insertion
          tit446
            		Point Mutation
            tp37
              		Point Mutation
              um13TgTransgenic Insertion
                um14TgTransgenic Insertion
                  um15TgTransgenic Insertion
                    um43TgTransgenic Insertion
                      1 - 10 of 10
                      Show
                      Human Disease / Model
                      No data available
                      Sequence Targeting Reagents
                      Target Reagent Reagent Type
                      dlcMO2-dlcMRPHLNO
                      dll4MO2-dll4MRPHLNO
                      notch1aMO4-notch1aMRPHLNO
                      notch1bMO6-notch1bMRPHLNO
                      1 - 4 of 4
                      Show
                      Fish
                      Fish
                      dlctit446/tit446
                      dlctit446/tit446; um14Tg + MO2-dll4
                      dll4j16e1/j16e1
                      jh12Tg
                      jh12Tg; jh15Tg
                      jh12Tg; um43Tg
                      jh15Tg
                      notch1atp37/tp37
                      notch3fh332/fh332
                      notch3fh332/fh332; um14Tg
                      1 - 10 of 23
                      Show
                      Antibodies
                      Name Type Antigen Genes Isotypes Host Organism
                      Ab1-etsrppolyclonalRabbit
                      Ab2-dlcmonoclonal
                        		IgG2aMouse
                        1 - 2 of 2
                        Show
                        Orthology
                        No data available
                        Engineered Foreign Genes
                        Marker Marker Type Name
                        CeruleanEFGCerulean
                        CreEFGCre
                        DsRedxEFGDsRedx
                        EGFPEFGEGFP
                        KaedeEFGKaede
                        mCherryEFGmCherry
                        1 - 6 of 6
                        Show
                        Mapping
                        No data available
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                        Citation
                        Quillien, A., Moore, J.C., Shin, M., Siekmann, A.F., Smith, T., Pan, L., Moens, C.B., Parsons, M.J., Lawson, N.D. (2014) Distinct Notch signaling outputs pattern the developing arterial system. Development (Cambridge, England). 141:1544-52.