PUBLICATION

Immunophilin FKBP52 induces Tau-P301L filamentous assembly in vitro and modulates its activity in a model of tauopathy

Authors
Giustiniani, J., Chambraud, B., Sardin, E., Dounane, O., Guillemeau, K., Nakatani, H., Paquet, D., Kamah, A., Landrieu, I., Lippens, G., Baulieu, E.E., Tawk, M.
ID
ZDB-PUB-140513-363
Date
2014
Source
Proceedings of the National Academy of Sciences of the United States of America   111: 4584-9 (Journal)
Registered Authors
Paquet, Dominik, Tawk, Marcel
Keywords
FKBP, Tau assembly, Tau-P301L dementia
MeSH Terms
  • Tacrolimus Binding Proteins/genetics
  • Tacrolimus Binding Proteins/metabolism
  • Tacrolimus Binding Proteins/physiology*
  • Zebrafish/physiology
  • Stereotyped Behavior
  • tau Proteins/metabolism
  • tau Proteins/physiology*
  • Motor Neurons/metabolism
  • Tauopathies/pathology*
  • Tauopathies/physiopathology
  • Humans
  • Cell Death/genetics
  • Animals
  • Cell Line
  • Phosphorylation
  • Animals, Genetically Modified
  • Biopolymers/metabolism
  • In Vitro Techniques
  • Gene Knockdown Techniques
  • Models, Biological*
(all 20)
PubMed
24623856 Full text @ Proc. Natl. Acad. Sci. USA
Abstract
The Tau protein is the major component of intracellular filaments observed in a number of neurodegenerative diseases known as tauopathies. The pathological mutant of Tau containing a proline-to-leucine mutation at position 301 (P301L) leads to severe human tauopathy. Here, we assess the impact of FK506-binding protein with a molecular mass of ~52 kDa (FKBP52), an immunophilin protein that interacts with physiological Tau, on Tau-P301L activity. We identify a direct interaction of FKBP52 with Tau-P301L and its phosphorylated forms and demonstrate FKBP52's ability to induce the formation of Tau-P301L oligomers. EM analysis shows that Tau-P301L oligomers, induced by FKBP52, can assemble into filaments. In the transgenic zebrafish expressing the human Tau-P301L mutant, FKBP52 knockdown is sufficient to redrive defective axonal outgrowth and branching related to Tau-P301L expression in spinal primary motoneurons. This result correlates with a significant reduction of pT181 pathological phosphorylated Tau and with recovery of the stereotypic escape response behavior. Collectively, FKBP52 appears to be an endogenous candidate that directly interacts with the pathogenic Tau-P301L and modulates its function in vitro and in vivo.
Genes / Markers
Figures
Figure Gallery (5 images)
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Expression
Phenotype
Mutations / Transgenics
Allele Construct Type Affected Genomic Region
knu3TgTransgenic Insertion
    mde3TgTransgenic Insertion
      1 - 2 of 2
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      Human Disease / Model
      No data available
      Sequence Targeting Reagents
      Target Reagent Reagent Type
      fkbp4MO1-fkbp4MRPHLNO
      1 - 1 of 1
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      Fish
      Antibodies
      Orthology
      Gene Orthology
      fkbp4
      1 - 1 of 1
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      Engineered Foreign Genes
      Marker Marker Type Name
      DsRedEFGDsRed
      EGFPEFGEGFP
      1 - 2 of 2
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      Mapping
      No data available