PUBLICATION

ErbB expressing Schwann cells control lateral line progenitor cells via non-cell-autonomous regulation of Wnt/beta-catenin

Authors

Lush, M.E., Piotrowski, T.

ID

ZDB-PUB-140513-325

Date

2014

Source

eLIFE 3: e01832 (Journal)

Registered Authors

Lush, Mark E., Piotrowski, Tatjana

Keywords

glia, neuromast, stem cells

MeSH Terms

Animals, Genetically Modified
Time Factors
Zebrafish Proteins/genetics
Zebrafish Proteins/metabolism*
Cell Differentiation
Protein Kinase Inhibitors/pharmacology
Genotype
Schwann Cells/drug effects
Schwann Cells/metabolism*
beta Catenin/genetics
beta Catenin/metabolism*
Gene Expression Regulation, Developmental
Neuregulins/metabolism
Wnt Signaling Pathway*/drug effects
Cell Communication*/drug effects
Mutation
Neural Stem Cells/drug effects
Neural Stem Cells/metabolism*
Animals
Stem Cell Niche
Zebrafish/genetics
Zebrafish/metabolism
Receptors, Notch/metabolism
Lateral Line System/cytology
Lateral Line System/drug effects
Lateral Line System/metabolism*
Fibroblast Growth Factors/metabolism
Phenotype
Cell Proliferation

(all 29)

PubMed

24642408 Full text @ Elife

Abstract

Proper orchestration of quiescence and activation of progenitor cells is crucial during embryonic development and adult homeostasis. We took advantage of the zebrafish sensory lateral line to define niche-progenitor interactions to understand how integration of diverse signaling pathways spatially and temporally regulates the coordination of these processes. Our previous studies demonstrated that Schwann cells play a crucial role in negatively regulating lateral line progenitor proliferation. Here we demonstrate that ErbB/Neuregulin signaling is not only required for Schwann cell migration but that it plays a continued role in postmigratory Schwann cells. ErbB expressing Schwann cells inhibit lateral line progenitor proliferation and differentiation through non-cell-autonomous inhibition of Wnt/β-catenin signaling. Subsequent activation of Fgf signaling controls sensory organ differentiation, but not progenitor proliferation. In addition to the lateral line, these findings have important implications for understanding how niche-progenitor cells segregate interactions during development, and how they may go wrong in disease states.

Genes / Markers

Figures

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Expression

Gene	Fish	Conditions	Stage	Anatomy	Assay	Figure
atoh1a	psi3Tg (TU)	control	Long-pec	interneuromast cell	ISH	Fig. 11
atoh1a	psi3Tg (TU)	chemical treatment: pharmaceutical	Long-pec	neuromast	ISH	Fig. 11
atoh1a	psi3Tg (TU)	control	Long-pec	neuromast	ISH	Fig. 11
atoh1a	WT	chemical treatment: pharmaceutical	Long-pec	neuromast	ISH	Fig. 11
atoh1a	WT	control	Long-pec	neuromast	ISH	Fig. 11
ctnnb2	nrg1^z26/z26	standard conditions	Long-pec	interneuromast cell	ISH	Fig. 4
ctnnb2	WT	standard conditions	Long-pec	interneuromast cell	ISH	Fig. 5
ctnnb2	WT	standard conditions	Day 4	interneuromast cell	ISH	Fig. 5
ctnnb2	WT	standard conditions	Protruding-mouth	interneuromast cell	ISH	Fig. 5
ctnnb2	WT	standard conditions	Long-pec	interneuromast cell	ISH	Fig. 4

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Phenotype

Fish	Conditions	Stage	Phenotype	Figure
WT	chemical treatment: pharmaceutical	Day 5	neuromast increased amount, abnormal	Fig. 1
nrg1^z26/z26	standard conditions	Long-pec	interneuromast cell canonical Wnt signaling pathway increased process quality, abnormal	Fig. 4
nrg1^z26/z26	standard conditions	Long-pec	interneuromast cell fibroblast growth factor receptor signaling pathway increased process quality, abnormal	Fig. 4
nrg1^z26/z26	standard conditions	Long-pec	interneuromast cell Notch signaling pathway increased process quality, abnormal	Fig. 4
nrg1^z26/z26	standard conditions	Day 5	myelinating Schwann cell decreased amount, abnormal	Fig. 1
nrg1^z26/z26	standard conditions	Day 5	neuromast increased amount, abnormal	Fig. 1
psi3Tg (TU)	control	Long-pec	neuromast increased amount, abnormal	Fig. 7
psi3Tg; sqet20Et	control	Long-pec	interneuromast cell cell population proliferation increased process quality, abnormal	Fig. 8
psi3Tg; sqet20Et	control	Long-pec	interneuromast cell cell population proliferation increased process quality, abnormal	Fig. 10
psi3Tg; sqet20Et	control	Long-pec	interneuromast cell increased amount, abnormal	Fig. 8

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Mutations / Transgenics

Allele	Construct	Type	Affected Genomic Region
kyu1Tg	Tg(OTM:d2EGFP)	Transgenic Insertion
nkhgn39dEt	Et(T2KHG)	Transgenic Insertion	cntnap2a
pd1Tg	Tg(hsp70l:dnfgfr1a-EGFP)	Transgenic Insertion
psi3Tg	Tg(-7.2sox10:dnHsa.ERBB4-RFP,myl7:EGFP)	Transgenic Insertion
sqet20Et	Et(krt4:EGFP)	Transgenic Insertion
st61		Point Mutation	erbb2
w32Tg	Tg(hsp70l:dkk1b-GFP)	Transgenic Insertion
z26		Point Mutation	nrg1
zd11		Small Deletion	lef1
zf15Tg	Tg(foxd3:GFP)	Transgenic Insertion

1 - 10 of 11

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Human Disease / Model

Sequence Targeting Reagents

Fish

Fish
cntnap2a^nkhgn39dEt
erbb2^st61/st61
kyu1Tg
lef1^zd11/zd11
nrg1^z26/z26
pd1Tg
psi3Tg; sqet20Et
psi3Tg; sqet20Et; w32Tg
psi3Tg (TU)
psi3Tg; w32Tg

Antibodies

Orthology

Engineered Foreign Genes

Marker	Marker Type	Name
d2EGFP	EFG	d2EGFP
EGFP	EFG	EGFP
GFP	EFG	GFP
RFP	EFG	RFP

Mapping

Lush et al., 2014 ZDB-PUB-140513-325 PMID:24642408

ErbB expressing Schwann cells control lateral line progenitor cells via non-cell-autonomous regulation of Wnt/beta-catenin

Lush et al., 2014

ZDB-PUB-140513-325
PMID:24642408