PUBLICATION

Differential regulation of myosin heavy chains defines new muscle domains in zebrafish

Authors

Nord, H., Burguiere, A.C., Muck, J., Nord, C., Ahlgren, U., and von Hofsten, J.

ID

ZDB-PUB-140502-13

Date

2014

Source

Molecular biology of the cell 25(8): 1384-95 (Journal)

Registered Authors

Burguière, Anne-Cécile, Muck, Joscha, Nord, Hanna, von Hofsten, Jonas

Keywords

none

MeSH Terms

Animals
Gene Expression
Tretinoin/pharmacology*
Myosin Heavy Chains/biosynthesis*
Myosin Heavy Chains/genetics
Gene Expression Regulation, Developmental
Tail/embryology
Protein Structure, Tertiary
Gene Expression Regulation
Wnt Signaling Pathway/drug effects*
Muscle Development/genetics
Zebrafish
Muscle Fibers, Fast-Twitch/cytology
Protein Isoforms/biosynthesis
Morpholinos

(all 15)

PubMed

24523292 Full text @ Mol. Biol. Cell

Abstract

Numerous muscle lineages are formed during myogenesis within both slow- and fast-specific cell groups. In this study, we show that six fast muscle–specific myosin heavy chain genes have unique expression patterns in the zebrafish embryo. The expression of tail-specific myosin heavy chain (fmyhc2.1) requires wnt signaling and is essential for fast muscle organization within the tail. Retinoic acid treatment results in reduced wnt signaling, which leads to loss of the fmyhc2.1 domain. Retinoic acid treatment also results in a shift of muscle identity within two trunk domains defined by expression of fmyhc1.2 and fmyhc1.3 in favor of the anteriormost myosin isoform, fmyhc1.2. In summary, we identify new muscle domains along the anteroposterior axis in the zebrafish that are defined by individual nonoverlapping, differentially regulated expression of myosin heavy chain isoforms.

Genes / Markers

Figures

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Expression

Gene	Fish	Conditions	Stage	Anatomy	Assay	Figure
GFP	umu153Tg	standard conditions	Days 30-44 to Days 45-89	anal fin musculature	IHC	Fig. 2
GFP	umu153Tg	standard conditions	Protruding-mouth	caudal fin somite	IHC	Fig. 2
GFP	umu153Tg	standard conditions	Days 30-44 to Days 45-89	caudal fin musculature	IHC	Fig. 2
GFP	umu153Tg	standard conditions	Adult	caudal fin musculature	IHC	Fig. 3
GFP	umu153Tg	standard conditions	Days 30-44 to Days 45-89	dorsal fin musculature	IHC	Fig. 2
GFP	umu153Tg	standard conditions	Days 30-44 to Days 45-89	extraocular musculature	IHC	Fig. 2
GFP	umu153Tg	standard conditions	Protruding-mouth	extraocular musculature	IHC	Fig. 2
GFP	umu153Tg	standard conditions	Adult	fast muscle cell lateral region	IHC	Fig. 3
GFP	umu153Tg	standard conditions	Protruding-mouth	hyohyoideus	IHC	Fig. 2
GFP	umu153Tg	standard conditions	Days 30-44 to Days 45-89	hyohyoideus	IHC	Fig. 2

1 - 10 of 169

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Phenotype

Fish	Conditions	Stage	Phenotype	Figure
umu153Tg	chemical treatment: pharmaceutical	Long-pec	caudal fin muscle cell malformed, abnormal	Fig. 5
umu153Tg	chemical treatment: pharmaceutical	Long-pec	caudal fin musculature sarcomere disorganized, abnormal	Fig. 5
umu153Tg	chemical treatment: pharmaceutical	Long-pec	muscle cell sarcomere disorganized, abnormal	Fig. 5
umu153Tg + MO1-myhz2	standard conditions	Prim-15	caudal fin muscle cell deformed, abnormal	Fig. 4
umu153Tg + MO1-myhz2	standard conditions	Prim-15	caudal fin muscle cell malformed, abnormal	Fig. 4
umu153Tg + MO1-myhz2	standard conditions	Prim-15	caudal fin musculature sarcomere disorganized, abnormal	Fig. 4
umu153Tg + MO1-myhz2	standard conditions	Prim-15	muscle cell deformed, abnormal	Fig. 4
umu153Tg + MO1-myhz2	standard conditions	Prim-15	muscle cell malformed, abnormal	Fig. 4
umu153Tg + MO1-myhz2	standard conditions	Prim-15	muscle cell sarcomere disorganized, abnormal	Fig. 4

1 - 9 of 9

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Mutations / Transgenics

Allele	Construct	Type	Affected Genomic Region
umu153Tg	Tg(myhz2:GFP)	Transgenic Insertion
umu160Tg	Tg(myhz1.2:GFP)	Transgenic Insertion

Human Disease / Model

Sequence Targeting Reagents

Target	Reagent	Reagent Type
myhz1.2	MO1-myhz1.2	MRPHLNO
myhz2	MO1-myhz2	MRPHLNO

Fish

Fish
umu153Tg
umu153Tg + MO1-myhz2
umu160Tg
umu160Tg + MO1-myhz1.2
WT

Antibodies

Orthology

Engineered Foreign Genes

Marker	Marker Type	Name
GFP	EFG	GFP

Mapping

Nord et al., 2014 ZDB-PUB-140502-13 PMID:24523292

Differential regulation of myosin heavy chains defines new muscle domains in zebrafish

Nord et al., 2014

ZDB-PUB-140502-13
PMID:24523292