PUBLICATION

Pleiotropic effects of Sox2 during the development of the zebrafish epithalamus

Authors

Pavlou, S., Astell, K., Kasioulis, I., Gakovic, M., Baldock, R., van Heyningen, V., and Coutinho, P.

ID

ZDB-PUB-140410-26

Date

2014

Source

PLoS One 9(1): e87546 (Journal)

Registered Authors

Coutinho, Pedro, van Heyningen, Veronica

Keywords

none

MeSH Terms

Matrix Metalloproteinases, Secreted/genetics
Matrix Metalloproteinases, Secreted/metabolism
Animals
Zebrafish Proteins/genetics
Zebrafish Proteins/metabolism*
Neurogenesis/physiology*
Pineal Gland/cytology
Pineal Gland/embryology*
Signal Transduction/physiology*
SOX Transcription Factors/genetics
SOX Transcription Factors/metabolism*
Gene Knockdown Techniques
Zebrafish/embryology*
Zebrafish/genetics

(all 14)

PubMed

24498133 Full text @ PLoS One

Abstract

The zebrafish epithalamus is part of the diencephalon and encompasses three major components: the pineal, the parapineal and the habenular nuclei. Using sox2 knockdown, we show here that this key transcriptional regulator has pleiotropic effects during the development of these structures. Sox2 negatively regulates pineal neurogenesis. Also, Sox2 is identified as the unknown factor responsible for pineal photoreceptor prepatterning and performs this function independently of the BMP signaling. The correct levels of sox2 are critical for the functionally important asymmetrical positioning of the parapineal organ and for the migration of parapineal cells as a coherent structure. Deviations from this strict control result in defects associated with abnormal habenular laterality, which we have documented and quantified in sox2 morphants.

Genes / Markers

Figures

Show all Figures

Expression

Gene	Fish	Conditions	Stage	Anatomy	Assay	Figure
EGFP	knu3Tg	chemical treatment: DAPT	Prim-5	pineal complex neuron	IHC	Fig. 5
EGFP	knu3Tg	control	Prim-5	pineal complex neuron	IHC	Fig. 4
EGFP	knu3Tg	control	Prim-5	pineal complex neuron	IHC	Fig. 5
EGFP	knu3Tg + MO6-sox2	standard conditions	Prim-5	pineal complex neuron	IHC	Fig. 4
EGFP	knu3Tg + MO6-sox2	standard conditions	Prim-5	pineal complex neuron	IHC	Fig. 5
EGFP	knu3Tg + MO6-sox2	chemical treatment: DAPT	Prim-5	pineal complex neuron	IHC	Fig. 5
EGFP	mw30Tg	control	Prim-5	pineal complex photoreceptor cell	IHC	Fig. 6
EGFP	mw30Tg + MO6-sox2	standard conditions	Prim-5	pineal complex photoreceptor cell	IHC	Fig. 6
EGFP	u711Tg	standard conditions	10-13 somites	pineal complex	IHC	Fig. 2
EGFP	u711Tg	standard conditions	Prim-5	pineal complex	IHC	Fig. 2

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Phenotype

Fish	Conditions	Stage	Phenotype	Figure
WT + MO6-sox2	standard conditions	Prim-5 to Day 4	determination of left/right asymmetry in diencephalon process quality, abnormal	Fig. 7
WT + MO6-sox2	standard conditions	Prim-5 to Day 4	habenula development process quality, abnormal	Fig. 7
WT + MO6-sox2	standard conditions	Prim-5 to Day 4	parapineal organ determination of left/right asymmetry in diencephalon process quality, abnormal	Fig. 7
WT + MO6-sox2	standard conditions	Prim-5 to Day 4	parapineal organ epithalamus development process quality, abnormal	Fig. 7
WT + MO6-sox2	standard conditions	Long-pec	eye decreased size, abnormal	Fig. 1
WT + MO6-sox2	standard conditions	Long-pec	whole organism decreased length, abnormal	Fig. 1
y8Tg	chemical treatment: pharmaceutical	Prim-5	pineal complex photoreceptor cell decreased amount, abnormal	Fig. S9
y8Tg + MO6-sox2	chemical treatment: DAPT	Prim-5	pineal complex photoreceptor cell increased amount, abnormal	Fig. 5
y8Tg + MO6-sox2	chemical treatment: dorsomorphin	Prim-5	pineal complex photoreceptor cell amount, normal	Fig. S9
y8Tg + MO6-sox2	standard conditions	Prim-5	pineal complex photoreceptor cell increased amount, abnormal	Fig. 4

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Mutations / Transgenics

Allele	Construct	Type
kca3Tg	Tg(5xUAS-E1B:6xMYC-notch1a)	Transgenic Insertion
kca4Tg	Tg(-1.5hsp70l:GAL4)	Transgenic Insertion
knu3Tg	Tg(elavl3:EGFP)	Transgenic Insertion
mw30Tg	Tg(BMPRE-AAV.Mlp:d2EGFP)	Transgenic Insertion
qmc61Tg	Tg(CSLBS:Venus)	Transgenic Insertion
u711Tg	Tg(-1.6flh:GAP-EGFP)	Transgenic Insertion
y8Tg	Tg(aanat2:EGFP)	Transgenic Insertion
zf15Tg	Tg(foxd3:GFP)	Transgenic Insertion

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Human Disease / Model

Sequence Targeting Reagents

Target	Reagent	Reagent Type
sox2	MO5-sox2,sox3	MRPHLNO
sox2	MO6-sox2	MRPHLNO
sox3	MO5-sox2,sox3	MRPHLNO

Fish

Fish
kca3Tg; kca4Tg
knu3Tg
knu3Tg + MO6-sox2
mw30Tg
mw30Tg + MO6-sox2
qmc61Tg
u711Tg
WT
WT + MO6-sox2
y8Tg

Antibodies

Orthology

Engineered Foreign Genes

Marker	Marker Type	Name
d2EGFP	EFG	d2EGFP
EGFP	EFG	EGFP
GAL4	EFG	GAL4
GFP	EFG	GFP
Venus	EFG	Venus

Mapping

Pavlou et al., 2014 ZDB-PUB-140410-26 PMID:24498133

Pleiotropic effects of Sox2 during the development of the zebrafish epithalamus

Pavlou et al., 2014

ZDB-PUB-140410-26
PMID:24498133