ADCK4 mutations promote steroid-resistant nephrotic syndrome through CoQ10 biosynthesis disruption
- Authors
- Ashraf, S., Gee, H.Y., Woerner, S., Xie, L.X., Vega-Warner, V., Lovric, S., Fang, H., Song, X., Cattran, D.C., Avila-Casado, C., Paterson, A.D., Nitschké, P., Bole-Feysot, C., Cochat, P., Esteve-Rudd, J., Haberberger, B., Allen, S.J., Zhou, W., Airik, R., Otto, E.A., Barua, M., Al-Hamed, M.H., Kari, J.A., Evans, J., Bierzynska, A., Saleem, M.A., Böckenhauer, D., Kleta, R., El Desoky, S., Hacihamdioglu, D.O., Gok, F., Washburn, J., Wiggins, R.C., Choi, M., Lifton, R.P., Levy, S., Han, Z., Salviati, L., Prokisch, H., Williams, D.S., Pollak, M., Clarke, C.F., Pei, Y., Antignac, C., and Hildebrandt, F.
- ID
- ZDB-PUB-140108-19
- Date
- 2013
- Source
- J. Clin. Invest. 123(12): 5179-5189 (Journal)
- Registered Authors
- Zhou, Weibin
- Keywords
- none
- MeSH Terms
-
- Sequence Alignment
- Adrenal Cortex Hormones/pharmacology
- Adrenal Cortex Hormones/therapeutic use
- Protein Kinases/deficiency
- Protein Kinases/genetics
- Protein Kinases/physiology*
- Exome/genetics
- Fibroblasts/metabolism
- Conserved Sequence
- Disease Models, Animal
- Mitochondria/physiology
- Zebrafish Proteins/antagonists & inhibitors
- Zebrafish Proteins/genetics
- Rats
- Drug Resistance
- Child
- Ubiquinone/analogs & derivatives*
- Ubiquinone/antagonists & inhibitors
- Ubiquinone/biosynthesis
- Ubiquinone/metabolism
- Ubiquinone/therapeutic use
- Molecular Sequence Data
- Young Adult
- Drosophila Proteins/antagonists & inhibitors
- Drosophila Proteins/genetics
- Consanguinity
- Zebrafish/embryology
- Zebrafish/genetics
- Animals
- Podocytes/metabolism
- Podocytes/ultrastructure
- Cells, Cultured
- Mutation
- Humans
- Sequence Homology, Amino Acid
- Amino Acid Sequence
- Nephrotic Syndrome/drug therapy
- Nephrotic Syndrome/genetics*
- Nephrotic Syndrome/metabolism
- Nephrotic Syndrome/pathology
- Adolescent
- Gene Knockdown Techniques
- DNA Mutational Analysis
- PubMed
- 24270420 Full text @ J. Clin. Invest.
Identification of single-gene causes of steroid-resistant nephrotic syndrome (SRNS) has furthered the understanding of the pathogenesis of this disease. Here, using a combination of homozygosity mapping and whole human exome resequencing, we identified mutations in the aarF domain containing kinase 4 (ADCK4) gene in 15 individuals with SRNS from 8 unrelated families. ADCK4 was highly similar to ADCK3, which has been shown to participate in coenzyme Q10 (CoQ10) biosynthesis. Mutations in ADCK4 resulted in reduced CoQ10 levels and reduced mitochondrial respiratory enzyme activity in cells isolated from individuals with SRNS and transformed lymphoblasts. Knockdown of adck4 in zebrafish and Drosophila recapitulated nephrotic syndrome-associated phenotypes. Furthermore, ADCK4 was expressed in glomerular podocytes and partially localized to podocyte mitochondria and foot processes in rat kidneys and cultured human podocytes. In human podocytes, ADCK4 interacted with members of the CoQ10 biosynthesis pathway, including COQ6, which has been linked with SRNS and COQ7. Knockdown of ADCK4 in podocytes resulted in decreased migration, which was reversed by CoQ10 addition. Interestingly, a patient with SRNS with a homozygous ADCK4 frameshift mutation had partial remission following CoQ10 treatment. These data indicate that individuals with SRNS with mutations in ADCK4 or other genes that participate in CoQ10 biosynthesis may be treatable with CoQ10.