PUBLICATION

Sdc2 and Tbx16 regulate Fgf2-dependent epithelial cell morphogenesis in the ciliated organ of asymmetry

Authors
Arrington, C.B., Peterson, A.G., and Yost, H.J.
ID
ZDB-PUB-130927-31
Date
2013
Source
Development (Cambridge, England)   140(19): 4102-4109 (Journal)
Registered Authors
Yost, H. Joseph
Keywords
Fgf2, Kupffer's vesicle, left-right patterning, Syndecan 2, Spadetail
MeSH Terms
  • Animals
  • Embryo, Nonmammalian/metabolism*
  • Zebrafish Proteins/genetics
  • Zebrafish Proteins/metabolism*
  • T-Box Domain Proteins/genetics
  • T-Box Domain Proteins/metabolism*
  • Cilia/metabolism*
  • Immunohistochemistry
  • Zebrafish/embryology*
  • Zebrafish/metabolism
  • Epithelial Cells/cytology*
  • Epithelial Cells/metabolism
  • Fibroblast Growth Factor 2/genetics
  • Fibroblast Growth Factor 2/metabolism*
  • In Situ Hybridization
  • Cell Differentiation/genetics
  • Cell Differentiation/physiology
  • Syndecan-2/genetics
  • Syndecan-2/metabolism*
(all 19)
PubMed
24046323 Full text @ Development
Abstract

Heparan sulfate proteoglycans (HSPGs) control many cellular processes and have been implicated in the regulation of left-right (LR) development by as yet unknown mechanisms. Using lineage-targeted knockdowns, we found that the transmembrane HSPG Syndecan 2 (Sdc2) regulates LR patterning through cell-autonomous functions in the zebrafish ciliated organ of asymmetry, Kupffer’s vesicle (KV), including regulation of cell proliferation and adhesion, cilia length and asymmetric fluid flow. Exploring downstream pathways, we found that the cell signaling ligand Fgf2 is exclusively expressed in KV cell lineages, and is dependent on Sdc2 and the transcription factor Tbx16. Strikingly, Fgf2 controls KV morphogenesis but not KV cilia length, and KV morphogenesis in sdc2 morphants can be rescued by expression of fgf2 mRNA. Through an Fgf2-independent pathway, Sdc2 and Tbx16 also control KV ciliogenesis. Our results uncover a novel Sdc2-Tbx16-Fgf2 pathway that regulates epithelial cell morphogenesis.

Genes / Markers
Figures
Figure Gallery (8 images)
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Expression
Phenotype
Mutations / Transgenics
Allele Construct Type Affected Genomic Region
b104
    Indel
    s870TgTransgenic Insertion
      1 - 2 of 2
      Show
      Human Disease / Model
      No data available
      Sequence Targeting Reagents
      Target Reagent Reagent Type
      fgf2MO1-fgf2MRPHLNO
      sdc2MO4-sdc2MRPHLNO
      sdc2MO5-sdc2MRPHLNO
      sdc2MO7-sdc2MRPHLNO
      tbx16MO3-tbx16MRPHLNO
      1 - 5 of 5
      Show
      Fish
      Antibodies
      Orthology
      No data available
      Engineered Foreign Genes
      Marker Marker Type Name
      GFPEFGGFP
      1 - 1 of 1
      Show
      Mapping
      No data available