PUBLICATION

Transcriptional components of anteroposterior positional information during zebrafish fin regeneration

Authors
Nachtrab, G., Kikuchi, K., Tornini, V.A., and Poss, K.D.
ID
ZDB-PUB-130903-1
Date
2013
Source
Development (Cambridge, England)   140(18): 3754-3764 (Journal)
Registered Authors
Kikuchi, Kazu, Nachtrab, Greg, Poss, Kenneth D., Tornini, Valerie A.
Keywords
regeneration, anteroposterior patterning, zebrafish, fin, blastema, Hand2, Vitamin D, positional memory
MeSH Terms
  • Gene Expression Regulation, Developmental
  • Male
  • Osteoblasts/metabolism
  • Vitamin D/metabolism
  • Signal Transduction/genetics
  • Animal Fins/growth & development*
  • Fibroblasts/metabolism
  • Transcription, Genetic*
  • Body Patterning/genetics*
  • Bone and Bones/anatomy & histology
  • Zebrafish Proteins/genetics
  • Zebrafish Proteins/metabolism
  • Models, Biological
  • Animals
  • Zebrafish/genetics*
  • Zebrafish/growth & development*
  • Regeneration/genetics*
  • Organ Specificity/genetics
  • Basic Helix-Loop-Helix Transcription Factors/genetics
  • Basic Helix-Loop-Helix Transcription Factors/metabolism
(all 20)
PubMed
23924636 Full text @ Development
Abstract

Many fish and salamander species regenerate amputated fins or limbs, restoring the size and shape of the original appendage. Regeneration requires that spared cells retain or recall information encoding pattern, a phenomenon termed positional memory. Few factors have been implicated in positional memory during vertebrate appendage regeneration. Here, we investigated potential regulators of anteroposterior (AP) pattern during fin regeneration in adult zebrafish. Sequence-based profiling from tissues along the AP axis of uninjured pectoral fins identified many genes with region-specific expression, several of which encoded transcription factors with known AP-specific expression or function in developing embryonic pectoral appendages. Transgenic reporter strains revealed that regulatory sequences of the transcription factor gene alx4a activated expression in fibroblasts and osteoblasts within anterior fin rays, whereas hand2 regulatory sequences activated expression in these same cell types within posterior rays. Transgenic overexpression of hand2 in all pectoral fin rays did not affect formation of the proliferative regeneration blastema, yet modified the lengths and widths of regenerating bones. Hand2 influenced the character of regenerated rays in part by elevation of the vitamin D-inactivating enzyme encoded by cyp24a1, contributing to region-specific regulation of bone metabolism. Systemic administration of vitamin D during regeneration partially rescued bone defects resulting from hand2 overexpression. Thus, bone-forming cells in a regenerating appendage maintain expression throughout life of transcription factor genes that can influence AP pattern, and differ across the AP axis in their expression signatures of these and other genes. These findings have implications for mechanisms of positional memory in vertebrate tissues.

Genes / Markers
Figures
Figure Gallery (8 images)
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Expression
No data available
Phenotype
No data available
Mutations / Transgenics
Allele Construct Type Affected Genomic Region
pd21TgTransgenic Insertion
    pd51TgTransgenic Insertion
      pd52TgTransgenic Insertion
        pd53TgTransgenic Insertion
          pd54TgTransgenic Insertion
            pd55TgTransgenic Insertion
              pd56TgTransgenic Insertion
                s6
                  Deficiency
                  1 - 8 of 8
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                  Human Disease / Model
                  No data available
                  Sequence Targeting Reagents
                  Target Reagent Reagent Type
                  hand2MO2-hand2MRPHLNO
                  1 - 1 of 1
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                  Fish
                  Antibodies
                  Name Type Antigen Genes Isotypes Host Organism
                  Ab1-tp63monoclonalIgG2aMouse
                  zns-5monoclonal
                    IgG1Mouse
                    1 - 2 of 2
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                    Orthology
                    No data available
                    Engineered Foreign Genes
                    Marker Marker Type Name
                    DsRed2EFGDsRed2
                    EGFPEFGEGFP
                    1 - 2 of 2
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                    Mapping
                    No data available