PUBLICATION

An exclusively mesodermal origin of fin mesenchyme demonstrates that zebrafish trunk neural crest does not generate ectomesenchyme

Authors
Lee, R.T., Knapik, E.W., Thiery, J.P., and Carney, T.J.
ID
ZDB-PUB-130709-13
Date
2013
Source
Development (Cambridge, England)   140(14): 2923-2932 (Journal)
Registered Authors
Carney, Tom, Knapik, Ela W., Lee, Raymond
Keywords
fin mesenchyme, dermomyotome, ectomesenchyme, fibroblast, neural crest
MeSH Terms
  • Mesoderm/cytology
  • Female
  • Male
  • Embryo, Nonmammalian/cytology
  • Embryo, Nonmammalian/metabolism
  • Animal Fins/cytology
  • Animal Fins/embryology*
  • Biological Evolution
  • Neural Crest/physiology*
  • Zebrafish/embryology*
  • Zebrafish/genetics
  • Fibroblasts/cytology
  • Animals
(all 13)
PubMed
23739134 Full text @ Development
Abstract

The neural crest is a multipotent stem cell population that arises from the dorsal aspect of the neural tube and generates both non-ectomesenchymal (melanocytes, peripheral neurons and glia) and ectomesenchymal (skeletogenic, odontogenic, cartilaginous and connective tissue) derivatives. In amniotes, only cranial neural crest generates both classes, with trunk neural crest restricted to non-ectomesenchyme. By contrast, it has been suggested that anamniotes might generate derivatives of both classes at all axial levels, with trunk neural crest generating fin osteoblasts, scale mineral-forming cells and connective tissue cells; however, this has not been fully tested. The cause and evolutionary significance of this cranial/trunk dichotomy, and its absence in anamniotes, are debated. Recent experiments have disputed the contribution of fish trunk neural crest to fin osteoblasts and scale mineral-forming cells. This prompted us to test the contribution of anamniote trunk neural crest to fin connective tissue cells. Using genetics-based lineage tracing in zebrafish, we find that these fin mesenchyme cells derive entirely from the mesoderm and that neural crest makes no contribution. Furthermore, contrary to previous suggestions, larval fin mesenchyme cells do not generate the skeletogenic cells of the adult fin, but persist to form fibroblasts associated with adult fin rays. Our data demonstrate that zebrafish trunk neural crest does not generate ectomesenchymal derivatives and challenge long-held ideas about trunk neural crest fate. These findings have important implications for the ontogeny and evolution of the neural crest.

Genes / Markers
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Phenotype
No data available
Mutations / Transgenics
Allele Construct Type Affected Genomic Region
ba2TgTransgenic Insertion
    cz1701TgTransgenic Insertion
      i150TgTransgenic Insertion
        i269TgTransgenic Insertion
          m188
            Unknown
            m610
              Point Mutation
              rk8TgTransgenic Insertion
                sq6TgTransgenic Insertion
                  sq7TgTransgenic Insertion
                    sq8TgTransgenic Insertion
                      1 - 10 of 16
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                      Human Disease / Model
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                      Sequence Targeting Reagents
                      No data available
                      Fish
                      Antibodies
                      Name Type Antigen Genes Isotypes Host Organism
                      Ab1-DsRedpolyclonalRabbit
                      Ab1-KaedepolyclonalRabbit
                      Ab1-paxmonoclonal
                        Mouse
                        Ab16-GFPpolyclonalIgYChicken
                        zns-5monoclonal
                          IgG1Mouse
                          1 - 5 of 5
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                          Engineered Foreign Genes
                          Marker Marker Type Name
                          CreEFGCre
                          EGFPEFGEGFP
                          GAL4EFGGAL4
                          GFPEFGGFP
                          KaedeEFGKaede
                          mCherryEFGmCherry
                          TomatoEFGTomato
                          1 - 7 of 7
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