PUBLICATION

Characterization of transgenic zebrafish lines that express GFP in the retina, pineal gland, olfactory bulb, hatching gland, and optic tectum

Authors
Fang, W., Bonaffini, S., Zou, J., Wang, X., Zhang, C., Tsujimura, T., Kawamura, S., and Wei, X.
ID
ZDB-PUB-130405-8
Date
2013
Source
Gene expression patterns : GEP   13(5-6): 150-9 (Journal)
Registered Authors
Kawamura, Shoji, Wei, Xiangyun
Keywords
Crumbs2b, olfactory system, transgenic zebrafish, green opsin promoter, photoreceptor, retina
MeSH Terms
  • Zebrafish/genetics
  • Zebrafish/metabolism*
  • Rod Opsins/analysis*
  • Photoreceptor Cells, Vertebrate/cytology*
  • Photoreceptor Cells, Vertebrate/metabolism
  • Superior Colliculi
  • Animals, Genetically Modified
  • Retina/cytology
  • Zebrafish Proteins/metabolism*
  • Membrane Proteins/metabolism*
  • Olfactory Bulb/cytology
  • Pineal Gland/cytology
  • Tissue Distribution
  • Green Fluorescent Proteins/analysis*
  • Animals
(all 15)
PubMed
23499733 Full text @ Gene Expr. Patterns
Abstract

Transgenic animals are powerful tools to study gene function invivo. Here we characterize several transgenic zebrafish lines that express green fluorescent protein (GFP) under the control of the LCRRH2-RH2-1 or LCRRH2-RH2-2 green opsin regulatory elements. Using confocal immunomicroscopy, stereo-fluorescence microscopy, and Western blotting, we show that the Tg(LCRRH2-RH2-1:GFP)pt112 and Tg(LCRRH2-RH2-2:GFP)pt115 transgenic zebrafish lines express GFP in the pineal gland and certain types of photoreceptors. In addition, some of these lines also express GFP in the hatching gland, optic tectum, or olfactory bulb. Some of the expression patterns differ significantly from previously published similar transgenic fish lines, making them useful tools for studying the development of the corresponding tissues and organs. In addition, the variations of GFP expression among different lines corroborate the notion that transgenic expression is often subjected to position effect, thus emphasizing the need for careful verification of expression patterns when transgenic animal models are utilized for research.

Genes / Markers
Figures
Figure Gallery (6 images)
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Expression
Phenotype
No data available
Mutations / Transgenics
Allele Construct Type Affected Genomic Region
pt112TgTransgenic Insertion
    pt115aTgTransgenic Insertion
      pt115bTgTransgenic Insertion
        pt115cTgTransgenic Insertion
          pt115iTgTransgenic Insertion
            pt115jTgTransgenic Insertion
              pt115kTgTransgenic Insertion
                1 - 7 of 7
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                Human Disease / Model
                No data available
                Sequence Targeting Reagents
                No data available
                Fish
                Fish
                pt112Tg
                pt115aTg
                pt115bTg
                pt115cTg
                pt115iTg
                pt115jTg
                pt115kTg
                1 - 7 of 7
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                Antibodies
                Orthology
                No data available
                Engineered Foreign Genes
                Marker Marker Type Name
                EGFPEFGEGFP
                GFPEFGGFP
                1 - 2 of 2
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                Mapping
                No data available
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                Citation
                Fang, W., Bonaffini, S., Zou, J., Wang, X., Zhang, C., Tsujimura, T., Kawamura, S., and Wei, X. (2013) Characterization of transgenic zebrafish lines that express GFP in the retina, pineal gland, olfactory bulb, hatching gland, and optic tectum. Gene expression patterns : GEP. 13(5-6):150-9.