PUBLICATION

Notochord vacuoles are lysosome-related organelles that function in axis and spine morphogenesis

Authors
Ellis, K., Bagwell, J., and Bagnat, M.
ID
ZDB-PUB-130322-23
Date
2013
Source
The Journal of cell biology   200(5): 667-679 (Journal)
Registered Authors
Bagnat, Michel
Keywords
none
MeSH Terms
  • Time Factors
  • Morphogenesis
  • Notochord/metabolism
  • Notochord/physiology*
  • Endocytosis
  • Zebrafish Proteins/metabolism
  • Axis, Cervical Vertebra/embryology
  • Axis, Cervical Vertebra/metabolism
  • Axis, Cervical Vertebra/physiology*
  • Zebrafish/embryology
  • Zebrafish/genetics
  • Zebrafish/metabolism
  • Zebrafish/physiology*
  • Gene Expression Regulation, Developmental
  • Humans
  • HEK293 Cells
  • Recombinant Fusion Proteins/metabolism
  • rab GTP-Binding Proteins/metabolism
  • Proton-Translocating ATPases
  • Time-Lapse Imaging
  • Microscopy, Confocal
  • Endosomes/metabolism
  • Protein Transport
  • Animals, Genetically Modified
  • Hydrogen-Ion Concentration
  • Spine/embryology
  • Spine/metabolism
  • Spine/physiology*
  • Cell Movement
  • Lysosomes/metabolism
  • Lysosomes/physiology*
  • Animals
  • Transfection
(all 33)
PubMed
23460678 Full text @ J. Cell Biol.
Abstract

The notochord plays critical structural and signaling roles during vertebrate development. At the center of the vertebrate notochord is a large fluid-filled organelle, the notochord vacuole. Although these highly conserved intracellular structures have been described for decades, little is known about the molecular mechanisms involved in their biogenesis and maintenance. Here we show that zebrafish notochord vacuoles are specialized lysosome-related organelles whose formation and maintenance requires late endosomal trafficking regulated by the vacuole-specific Rab32a and H-ATPase-dependent acidification. We establish that notochord vacuoles are required for body axis elongation during embryonic development and identify a novel role in spine morphogenesis. Thus, the vertebrate notochord plays important structural roles beyond early development.

Genes / Markers
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Expression
Phenotype
Mutations / Transgenics
Allele Construct Type Affected Genomic Region
hi577aTgTransgenic Insertion
hi1207TgTransgenic Insertion
hi1988TgTransgenic Insertion
hi2462TgTransgenic Insertion
hi2499aTgTransgenic Insertion
kca3TgTransgenic Insertion
    kca33TgTransgenic Insertion
      mw33TgTransgenic Insertion
        mw35TgTransgenic Insertion
          mw37TgTransgenic Insertion
            1 - 10 of 22
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            Human Disease / Model
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            Sequence Targeting Reagents
            No data available
            Fish
            Antibodies
            Orthology
            Engineered Foreign Genes
            Marker Marker Type Name
            EGFPEFGEGFP
            GAL4EFGGAL4
            GAL4FFEFGGAL4FF
            GFPEFGGFP
            mCherryEFGmCherry
            RFPEFGRFP
            sGFPEFGsGFP
            1 - 7 of 7
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