PUBLICATION

Fibronectin mediates correct positioning of the interrenal organ in zebrafish

Authors
Chou, C.W., Chiu, C.H., and Liu, Y.W.
ID
ZDB-PUB-130211-6
Date
2013
Source
Developmental Dynamics : an official publication of the American Association of Anatomists   242(5): 432-443 (Journal)
Registered Authors
Liu, Yi-wen
Keywords
zebrafish, adrenal, interrenal, steroidogenic, chromaffin, fibronectin
MeSH Terms
  • Body Patterning/genetics*
  • Organogenesis/genetics
  • Fibronectins/genetics
  • Fibronectins/metabolism
  • Fibronectins/physiology*
  • Zebrafish Proteins/genetics
  • Zebrafish Proteins/metabolism
  • Animals, Genetically Modified
  • Chromaffin Cells/metabolism
  • Chromaffin Cells/physiology
  • Interrenal Gland/embryology*
  • Interrenal Gland/metabolism
  • Embryo, Nonmammalian
  • Transcription Factors/genetics
  • Transcription Factors/metabolism
  • Animals
  • Zebrafish/embryology*
  • Zebrafish/genetics
  • Zebrafish/metabolism
  • 3-Hydroxysteroid Dehydrogenases/genetics
  • 3-Hydroxysteroid Dehydrogenases/metabolism
  • Green Fluorescent Proteins/genetics
  • Green Fluorescent Proteins/metabolism
  • Gene Expression Regulation, Enzymologic
  • Cell Movement/genetics
  • Gene Expression Regulation, Developmental
(all 26)
PubMed
23362214 Full text @ Dev. Dyn.
Abstract

Background:

Fibronectin (Fn) forms a centripetal gradient during the fetal adrenal gland organogenesis, and modulates hormone responsiveness of adrenocortical cells in the primary culture. However, how Fn is involved in organ formation of the adrenal gland remains unclear.

Results:

In this study, we found that Fn accumulates around migrating ff1b-expressing interrenal cells which were marked by the ff1b promoter-driven transgenic fluorescence, during the course of interrenal organ assembly. The interrenal cells displaying migratory phenotype were absent in the fn1 mutant, while specification and kidney association of the interrenal tissue remained normal. The Fn deposition in the interrenal microenvironment was severely reduced in the vessel-deficient ets1b morphant, implying its origin of synthesis from the peri-interrenal vasculature. In the fn1 mutant, early-migrating chromaffin cells were capable of interacting with steroidogenic interrenal cells, yet continuous migration and midline convergence of chromaffin cells were disrupted. Migration defects of both interrenal and chromaffin lineages, in the absence of Fn, thus led to incomplete interrenal organ assembly in aberrant positions.

Conclusions:

Our results indicate that Fn is essential for patterning interrenal organ formation, by modulating the migratory behavior of both steroidogenic interrenal and chromaffin cells.

Genes / Markers
Figures
Figure Gallery (6 images)
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Expression
Phenotype
Mutations / Transgenics
Allele Construct Type Affected Genomic Region
kt259
    Point Mutation
    s843TgTransgenic Insertion
      y1TgTransgenic Insertion
        zf346TgTransgenic Insertion
          1 - 4 of 4
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          Human Disease / Model
          No data available
          Sequence Targeting Reagents
          Target Reagent Reagent Type
          etsrpMO1-etsrpMRPHLNO
          etsrpMO2-etsrpMRPHLNO
          1 - 2 of 2
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          Fish
          Antibodies
          Name Type Antigen Genes Isotypes Host Organism
          Ab1-ptk2polyclonalIgGRabbit
          Ab2-acta2polyclonalIgGRabbit
          Ab2-fnpolyclonal
            Rabbit
            1 - 3 of 3
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            Orthology
            No data available
            Engineered Foreign Genes
            Marker Marker Type Name
            EGFPEFGEGFP
            1 - 1 of 1
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            Mapping
            No data available