PUBLICATION

Inhibition of the pim1 oncogene results in diminished visual function

Authors
Yin, J., Shine, L., Raycroft, F., Deeti, S., Reynolds, A., Ackerman, K.M., Glaviano, A., O'Farrell, S., O'Leary, O., Kilty, C., Kennedy, C., McLoughlin, S., Rice, M., Russell, E., Higgins, D.G., Hyde, D.R., and Kennedy, B.N.
ID
ZDB-PUB-130118-6
Date
2012
Source
PLoS One   7(12): e52177 (Journal)
Registered Authors
Hyde, David R., Kennedy, Breandan N., McLoughlin, Sarah, Yin, Jun
Keywords
none
Datasets
GEO:GSE19320
MeSH Terms
  • Electroretinography
  • RNA, Messenger/genetics
  • Signal Transduction/drug effects
  • Suppressor of Cytokine Signaling Proteins/genetics
  • Suppressor of Cytokine Signaling Proteins/metabolism
  • Phylogeny
  • Morpholinos/pharmacology*
  • Real-Time Polymerase Chain Reaction
  • Larva/drug effects
  • Larva/growth & development
  • Larva/metabolism
  • Blotting, Western
  • Reverse Transcriptase Polymerase Chain Reaction
  • Zebrafish/genetics
  • Zebrafish/growth & development
  • Zebrafish/metabolism*
  • STAT3 Transcription Factor/genetics
  • STAT3 Transcription Factor/metabolism
  • Vision Disorders/drug therapy
  • Vision Disorders/etiology*
  • Vision Disorders/pathology
  • Gene Expression Regulation, Developmental*
  • Proto-Oncogene Proteins c-pim-1/antagonists & inhibitors*
  • Proto-Oncogene Proteins c-pim-1/genetics
  • Proto-Oncogene Proteins c-pim-1/metabolism
  • Zebrafish Proteins/genetics
  • Zebrafish Proteins/metabolism
  • Animals
(all 28)
PubMed
23300608 Full text @ PLoS One
Abstract

Our objective was to profile genetic pathways whose differential expression correlates with maturation of visual function in zebrafish. Bioinformatic analysis of transcriptomic data revealed Jak-Stat signalling as the pathway most enriched in the eye, as visual function develops. Real-time PCR, western blotting, immunohistochemistry and in situ hybridization data confirm that multiple Jak-Stat pathway genes are up-regulated in the zebrafish eye between 3–5 days post-fertilisation, times associated with significant maturation of vision. One of the most up-regulated Jak-Stat genes is the proto-oncogene Pim1 kinase, previously associated with haematological malignancies and cancer. Loss of function experiments using Pim1 morpholinos or Pim1 inhibitors result in significant diminishment of visual behaviour and function. In summary, we have identified that enhanced expression of Jak-Stat pathway genes correlates with maturation of visual function and that the Pim1 oncogene is required for normal visual function.

Genes / Markers
Figures
Figure Gallery (6 images)
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Expression
Phenotype
Mutations / Transgenics
Allele Construct Type Affected Genomic Region
nt11TgTransgenic Insertion
    1 - 1 of 1
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    Human Disease / Model
    No data available
    Sequence Targeting Reagents
    Target Reagent Reagent Type
    pim1MO1-pim1MRPHLNO
    pim1MO2-pim1MRPHLNO
    socs1aMO3-socs1aMRPHLNO
    socs3aMO5-socs3aMRPHLNO
    stat3MO3-stat3MRPHLNO
    1 - 5 of 5
    Show
    Fish
    Antibodies
    Orthology
    Gene Orthology
    pim1
    pim2
    pim3
    1 - 3 of 3
    Show
    Engineered Foreign Genes
    Marker Marker Type Name
    EGFPEFGEGFP
    1 - 1 of 1
    Show
    Mapping
    No data available