PUBLICATION

Regulated tissue fluidity steers zebrafish body elongation

Authors
Lawton, A.K., Nandi, A., Stulberg, M.J., Dray, N., Sneddon, M.W., Pontius, W., Emonet, T., and Holley, S.A.
ID
ZDB-PUB-130110-28
Date
2013
Source
Development (Cambridge, England)   140(3): 573-582 (Journal)
Registered Authors
Dray, Nicolas, Holley, Scott
Keywords
zebrafish, tailbud, body elongation, cell migration
MeSH Terms
  • Cadherins/genetics
  • Cadherins/metabolism
  • Cell Polarity
  • Embryonic Development
  • Zebrafish/embryology*
  • Zebrafish/genetics
  • Zebrafish/metabolism
  • Animals, Genetically Modified
  • Cell Count
  • Gene Expression Regulation, Developmental*
  • Cell Movement*
  • Tail/embryology
  • Tail/metabolism
  • Cell Adhesion
  • Wnt Signaling Pathway
  • Embryo, Nonmammalian/cytology
  • Embryo, Nonmammalian/metabolism
  • Zebrafish Proteins/genetics
  • Zebrafish Proteins/metabolism
  • Fibroblast Growth Factors/genetics
  • Fibroblast Growth Factors/metabolism
  • Time Factors
  • Body Patterning*
  • Computer Simulation
  • Models, Biological
  • Biomechanical Phenomena
  • Animals
(all 27)
PubMed
23293289 Full text @ Development
Abstract

The tailbud is the posterior leading edge of the growing vertebrate embryo and consists of motile progenitors of the axial skeleton, musculature and spinal cord. We measure the 3D cell flow field of the zebrafish tailbud and identify changes in tissue fluidity revealed by reductions in the coherence of cell motion without alteration of cell velocities. We find a directed posterior flow wherein the polarization between individual cell motion is high, reflecting ordered collective migration. At the posterior tip of the tailbud, this flow makes sharp bilateral turns facilitated by extensive cell mixing due to increased directional variability of individual cell motions. Inhibition of Wnt or Fgf signaling or cadherin 2 function reduces the coherence of the flow but has different consequences for trunk and tail extension. Modeling and additional data analyses suggest that the balance between the coherence and rate of cell flow determines whether body elongation is linear or whether congestion forms within the flow and the body axis becomes contorted.

Genes / Markers
Figures
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Expression
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Phenotype
Mutations / Transgenics
Allele Construct Type Affected Genomic Region
tm101
    Point Mutation
    w26TgTransgenic Insertion
      1 - 2 of 2
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      Human Disease / Model
      No data available
      Sequence Targeting Reagents
      No data available
      Fish
      1 - 2 of 2
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      Antibodies
      No data available
      Orthology
      No data available
      Engineered Foreign Genes
      Marker Marker Type Name
      GFPEFGGFP
      1 - 1 of 1
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      Mapping
      No data available