PUBLICATION

Structural disorganization of pronephric glomerulus in zebrafish mpp5a/nagie oko mutant

Authors
Ichimura, K., Fukuyo, Y., Nakamura, T., Powell, R., Sakai, T., and Obara, T.
ID
ZDB-PUB-121012-5
Date
2012
Source
Developmental Dynamics : an official publication of the American Association of Anatomists   241(12): 1922-1932 (Journal)
Registered Authors
Obara, Tomoko
Keywords
glomerulogenesis, pronephros, glomerulus, podocyte, slit diaphragm, mpp5a, nagie oko, Danio rerio
MeSH Terms
  • Guanylate Cyclase/genetics
  • Guanylate Cyclase/metabolism*
  • Podocytes/metabolism*
  • Glomerular Mesangium/embryology*
  • Mutation*
  • Heart/embryology
  • Zebrafish/embryology*
  • Zebrafish/genetics
  • Animals
  • Gene Knockdown Techniques
  • Troponin T/genetics
  • Troponin T/metabolism
  • Zebrafish Proteins/genetics
  • Zebrafish Proteins/metabolism*
(all 14)
PubMed
23027442 Full text @ Dev. Dyn.
Abstract

Background:

The podocyte slit diaphragm (SD) is an essential component of the selective filtration barrier in the glomerulus. Several structural proteins required for formation and maintenance of SD have been identified; however, molecular mechanisms regulating these proteins are still limited.

Here, we demonstrate that MAGUK p55 subfamily member 5a (Mpp5a)/Nagie oko, a component of the Crb multi-protein complex, was colocalized with an SD-associated protein ZO-1 in the zebrafish pronephric glomerulus. To characterize the function of Mpp5a, zebrafish mpp5am520 mutant embryos, which are known to have defects in cardiac and neuronal morphogenesis, were analyzed. These mutants failed to merge the bilateral glomerular primordia and to form the glomerular capillary and mesangium, but the foot processes and SD showed normal appearance. The structural disorganization in the mpp5am520 mutant glomerulus was quite similar to that of a cardiac troponin T2a/tnnt2a/silent heart knockdown zebrafish, which exhibited circulatory failure due to lack of heart beating.

Mpp5a is not prerequisite to form podocyte slit diaphragm in the pronephric glomerular development in zebrafish. The structural disorganization of the pronephric glomerulus in the mpp5am520 mutant is likely to result from circulatory failure, rather than the anomaly of Mpp5a protein in the glomerulus.

Genes / Markers
Figures
Figure Gallery (6 images)
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Expression
Phenotype
Mutations / Transgenics
Allele Construct Type Affected Genomic Region
m520
    Point Mutation
    1 - 1 of 1
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    Human Disease / Model
    No data available
    Sequence Targeting Reagents
    Target Reagent Reagent Type
    tnnt2aMO1-tnnt2aMRPHLNO
    1 - 1 of 1
    Show
    Fish
    1 - 3 of 3
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    Antibodies
    Name Type Antigen Genes Isotypes Host Organism
    Ab3-pals1apolyclonalRabbit
    1 - 1 of 1
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    Orthology
    No data available
    Engineered Foreign Genes
    No data available
    Mapping
    No data available