PUBLICATION

Long-Range Ca(2+) Waves Transmit Brain-Damage Signals to Microglia

Authors
Sieger, D., Moritz, C., Ziegenhals, T., Prykhozhij, S., and Peri, F.
ID
ZDB-PUB-120529-46
Date
2012
Source
Developmental Cell   22(6): 1138-1148 (Journal)
Registered Authors
Peri, Francesca, Prykhozhij, Sergey, Sieger, Dirk
Keywords
none
MeSH Terms
  • Chelating Agents/metabolism
  • Glutamic Acid/physiology
  • Animals
  • Zebrafish/growth & development
  • Zebrafish/physiology
  • Calcium Signaling/drug effects
  • Calcium Signaling/physiology*
  • Laser Therapy
  • Microglia/drug effects
  • Microglia/physiology*
  • Cell Death/drug effects
  • Cell Death/physiology
  • Adenosine Triphosphate/physiology
  • Cell Movement/drug effects
  • Cell Movement/physiology
  • Brain Injuries/physiopathology*
(all 16)
PubMed
22632801 Full text @ Dev. Cell
Abstract

Microglia are the resident phagocytes of the brain that are responsible for the clearance of injured neurons, an essential step in subsequent tissue regeneration. How death signals are controlled both in space and time to attract these cells toward the site of injury is a topic of great interest. To this aim, we have used the optically transparent zebrafish larval brain and identified rapidly propagating Ca2+ waves that determine the range of microglial responses to neuronal cell death. We show that while Ca2+-mediated microglial responses require ATP, the spreading of intercellular Ca2+ waves is ATP independent. Finally, we identify glutamate as a potent inducer of Ca2+-transmitted microglial attraction. Thus, this real-time analysis reveals the existence of a mechanism controlling microglial targeted migration to neuronal injuries that is initiated by glutamate and proceeds across the brain in the form of a Ca2+ wave.

Genes / Markers
Figures
Figure Gallery (9 images)
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Expression
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay Figure
DsRedzf148Tg; zf149Tgstandard conditionsPec-finIFL
DsRedzf148Tg; zf149Tgstandard conditionsPec-finIFL
DsRedzf148Tg; zf149Tgphysical alteration: anatomical structurePec-finIFL
DsRedzf148Tg; zf149Tg + MO1-duoxphysical alteration: anatomical structurePec-finIFL
DsRedzf148Tg; zf149Tg + MO1-p2ry12physical alteration: anatomical structurePec-finIFL
DsRedzf148Tg; zf149Tg + MO2-p2ry12physical alteration: anatomical structurePec-finIFL
EGFPzf148Tg; zf149Tgstandard conditionsPec-finIFL
EGFPzf148Tg; zf149Tgstandard conditionsPec-finIFL
EGFPzf148Tg; zf149Tgphysical alteration: anatomical structurePec-finIFL
EGFPzf148Tg; zf149Tg + MO1-duoxphysical alteration: anatomical structurePec-finIFL
1 - 10 of 17
Show
Phenotype
Fish Conditions Stage Phenotype Figure
zf148Tg; zf149Tg + MO1-p2ry12physical alteration: anatomical structurePec-fin
zf148Tg; zf149Tg + MO1-p2ry12physical alteration: anatomical structurePec-fin
zf148Tg; zf149Tg + MO2-p2ry12physical alteration: anatomical structurePec-fin
zf148Tg; zf149Tg + MO2-p2ry12physical alteration: anatomical structurePec-fin
1 - 4 of 4
Show
Mutations / Transgenics
Allele Construct Type Affected Genomic Region
hdb1TgTransgenic Insertion
    hdb2TgTransgenic Insertion
      hdb3TgTransgenic Insertion
        y1TgTransgenic Insertion
          zf148TgTransgenic Insertion
            zf149TgTransgenic Insertion
              1 - 6 of 6
              Show
              Human Disease / Model
              No data available
              Sequence Targeting Reagents
              Target Reagent Reagent Type
              duoxMO1-duoxMRPHLNO
              p2ry12MO1-p2ry12MRPHLNO
              p2ry12MO2-p2ry12MRPHLNO
              1 - 3 of 3
              Show
              Fish
              Fish
              hdb1Tg
              hdb1Tg; hdb2Tg
              hdb2Tg
              hdb2Tg; hdb3Tg
              hdb3Tg; zf149Tg
              y1Tg
              zf148Tg
              zf148Tg; zf149Tg
              zf148Tg; zf149Tg + MO1-duox
              zf148Tg; zf149Tg + MO1-p2ry12
              1 - 10 of 12
              Show
              Antibodies
              No data available
              Orthology
              No data available
              Engineered Foreign Genes
              Marker Marker Type Name
              DsRedEFGDsRed
              EGFPEFGEGFP
              GAL4EFGGAL4
              GCaMPEFGGCaMP
              GFPEFGGFP
              TagRFPEFGTagRFP
              1 - 6 of 6
              Show
              Mapping
              No data available
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              Citation
              Sieger, D., Moritz, C., Ziegenhals, T., Prykhozhij, S., and Peri, F. (2012) Long-Range Ca(2+) Waves Transmit Brain-Damage Signals to Microglia. Developmental Cell. 22(6):1138-1148.