PUBLICATION

Zebrafish globin switching occurs in two developmental stages and is controlled by the LCR

Authors
Ganis, J.J., Hsia, N., Trompouki, E., de Jong, J.L., Dibiase, A., Lambert, J.S., Jia, Z., Sabo, P.J., Weaver, M., Sandstrom, R., Stamatoyannopoulos, J.A., Zhou, Y., and Zon, L.I.
ID
ZDB-PUB-120430-12
Date
2012
Source
Developmental Biology   366(2): 185-194 (Journal)
Registered Authors
de Jong, Jill, Hsia, Nelson, Zhou, Yi, Zon, Leonard I.
Keywords
zebrafish, erythropoiesis, glopbin switching, locus control region
Datasets
GEO:GSE35895, GEO:GSE35875, GEO:GSE35874
MeSH Terms
  • NF-E2 Transcription Factor, p45 Subunit/genetics
  • Zebrafish Proteins/genetics
  • Locus Control Region/genetics*
  • Zebrafish/embryology
  • Zebrafish/genetics*
  • Genes, Switch
  • Gene Expression Regulation, Developmental
  • Animals
  • GATA1 Transcription Factor/genetics
  • Embryo, Nonmammalian/embryology
  • Embryo, Nonmammalian/physiology
  • Globins/genetics*
(all 12)
PubMed
22537494 Full text @ Dev. Biol.
Abstract

Globin gene switching is a complex, highly regulated process allowing expression of distinct globin genes at specific developmental stages. Here, for the first time, we have characterized all of the zebrafish globins based on the completed genomic sequence. Two distinct chromosomal loci, termed major (chromosome 3) and minor (chromosome 12), harbor the globin genes containing α/β pairs in a 52–32 to 32–52 orientation. Both these loci share synteny with the mammalian α-globin locus. Zebrafish globin expression was assayed during development and demonstrated two globin switches, similar to human development. A conserved regulatory element, the locus control region (LCR), was revealed by analyzing DNase I hypersensitive sites, H3K4 trimethylation marks and GATA1 binding sites. Surprisingly, the position of these sites with relation to the globin genes is evolutionarily conserved, despite a lack of overall sequence conservation. Motifs within the zebrafish LCR include CACCC, GATA, and NFE2 sites, suggesting functional interactions with known transcription factors but not the same LCR architecture. Functional homology to the mammalian α-LCR MCS-R2 region was confirmed by robust and specific reporter expression in erythrocytes of transgenic zebrafish. Our studies provide a comprehensive characterization of the zebrafish globin loci and clarify the regulation of globin switching.

Genes / Markers
Figures
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Expression
Phenotype
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Mutations / Transgenics
Allele Construct Type Affected Genomic Region
cz3325TgTransgenic Insertion
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    Human Disease / Model
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    Sequence Targeting Reagents
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    Orthology
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    Engineered Foreign Genes
    Marker Marker Type Name
    EGFPEFGEGFP
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    Mapping
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