PUBLICATION

Analysis of Sphingosine-1-phosphate signaling mutants reveals endodermal requirements for the growth but not dorsoventral patterning of jaw skeletal precursors

Authors
Balczerski, B., Matsutani, M., Castillo, P., Osborne, N., Stainier, D.Y., and Crump, J.G.
ID
ZDB-PUB-120106-5
Date
2012
Source
Developmental Biology   361(2): 230-241 (Journal)
Registered Authors
Balczerski, Bartosz, Crump, Gage DeKoeyer, Matsutani, Megan, Osborne, Nick, Stainier, Didier
Keywords
Sphingosine-1-phosphate, pharyngeal endoderm, facial ectoderm, craniofacial skeleton, Shh, zebrafish
MeSH Terms
  • DNA Primers/genetics
  • Jaw/embryology*
  • Animals
  • Hedgehog Proteins/metabolism
  • In Situ Hybridization
  • Craniofacial Abnormalities/genetics*
  • Zebrafish/embryology*
  • Receptors, Lysosphingolipid/genetics*
  • Receptors, Lysosphingolipid/metabolism
  • Genotype
  • Analysis of Variance
  • Zebrafish Proteins/genetics
  • Zebrafish Proteins/metabolism
  • Microscopy, Fluorescence
  • Membrane Proteins/genetics
  • Membrane Proteins/metabolism
  • Lysophospholipids/metabolism*
  • Signal Transduction/genetics*
  • Mutation/genetics
  • DNA, Complementary/genetics
  • Fibroblast Growth Factors/metabolism
  • Image Interpretation, Computer-Assisted
  • Carrier Proteins/genetics
  • Carrier Proteins/metabolism
  • Sphingosine/analogs & derivatives*
  • Sphingosine/metabolism
  • Endoderm/metabolism*
  • Body Patterning/genetics
  • Body Patterning/physiology
(all 29)
PubMed
22185793 Full text @ Dev. Biol.
Abstract

Development of the head skeleton involves reciprocal interactions between cranial neural crest cells (CNCCs) and the surrounding pharyngeal endoderm and ectoderm. Whereas elegant experiments in avians have shown a prominent role for the endoderm in facial skeleton development, the relative functions of the endoderm in growth versus regional identity of skeletal precursors have remained unclear. Here we describe novel craniofacial defects in zebrafish harboring mutations in the Sphingosine-1-phospate (S1P) type 2 receptor (s1pr2) or the S1P transporter Spinster 2 (spns2), and we show that S1P signaling functions in the endoderm for the proper growth and positioning of the jaw skeleton. Surprisingly, analysis of s1pr2 and spns2 mutants, as well as sox32 mutants that completely lack endoderm, reveals that the dorsal–ventral (DV) patterning of jaw skeletal precursors is largely unaffected even in the absence of endoderm. Instead, we observe reductions in the ectodermal expression of Fibroblast growth factor 8a (Fgf8a), and transgenic misexpression of Shha restores fgf8a expression and partially rescues the growth and differentiation of jaw skeletal precursors. Hence, we propose that the S1P-dependent anterior foregut endoderm functions primarily through Shh to regulate the growth but not DV patterning of zebrafish jaw precursors.

Genes / Markers
Figures
Figure Gallery (6 images)
Show all Figures
Expression
Phenotype
Mutations / Transgenics
Allele Construct Type Affected Genomic Region
b1110
    Point Mutation
    el2TgTransgenic Insertion
      el106TgTransgenic Insertion
        el137TgTransgenic Insertion
          kca4TgTransgenic Insertion
            m93
              Point Mutation
              ne1911TgTransgenic Insertion
                sk12
                  Unknown
                  ta56
                    Point Mutation
                    y1TgTransgenic Insertion
                      1 - 10 of 10
                      Show
                      Human Disease / Model
                      No data available
                      Sequence Targeting Reagents
                      No data available
                      Fish
                      Antibodies
                      No data available
                      Orthology
                      No data available
                      Engineered Foreign Genes
                      Marker Marker Type Name
                      EGFPEFGEGFP
                      GAL4EFGGAL4
                      KikGREFGKikGR
                      1 - 3 of 3
                      Show
                      Mapping
                      No data available