PUBLICATION

Analysis of Pax7 expressing myogenic cells in zebrafish muscle development, injury, and models of disease

Authors
Seger, C., Hargrave, M., Wang, X., Chai, R.J., Elworthy, S., and Ingham, P.W.
ID
ZDB-PUB-111013-7
Date
2011
Source
Developmental Dynamics : an official publication of the American Association of Anatomists   240(11): 2440-51 (Journal)
Registered Authors
Elworthy, Stone, Hargrave, Murray, Ingham, Philip, Seger, Claudia, Wang, Xingang
Keywords
Pax7, Pax3, Myod, GFP-reporter, dystrophin, integrin alpha7, muscle progenitor cell, satellite cell, muscle injury, repair, zebrafish
MeSH Terms
  • Muscle Development/genetics*
  • Humans
  • Embryo, Nonmammalian
  • Genes, Reporter/physiology
  • Animals
  • Muscle, Skeletal/injuries
  • Muscle, Skeletal/metabolism
  • Animals, Genetically Modified
  • Muscular Dystrophy, Duchenne/genetics
  • Muscular Dystrophy, Duchenne/metabolism
  • Muscular Dystrophy, Duchenne/pathology
  • PAX7 Transcription Factor/genetics*
  • PAX7 Transcription Factor/metabolism
  • Zebrafish/embryology*
  • Zebrafish/genetics
  • Zebrafish/metabolism
  • Disease Models, Animal*
  • Myoblasts/metabolism*
  • Myoblasts/physiology
  • Gene Expression Regulation, Developmental
  • Cobra Cardiotoxin Proteins
  • Muscular Diseases/chemically induced
  • Muscular Diseases/genetics*
  • Muscular Diseases/metabolism
  • Muscular Diseases/pathology
(all 25)
PubMed
21954137 Full text @ Dev. Dyn.
Abstract
The transcription factor Pax7 is a marker and regulator of muscle progenitors and satellite cells that contribute to the embryonic development and postembryonic growth of skeletal muscle in vertebrates, as well as to its repair and regeneration. Here, we identify Pax7+ve myogenic cells in the zebrafish and characterize their behavior in postembryonic stages. Mononucleate Pax7+ve cells can first be found associated with myofibers at 72 hours post fertilization (hpf). To follow the behavior of muscle progenitor cells in vivo, we generated transgenic lines expressing fluorescent proteins under the control of the pax7a or pax3a promoters. We established an injury model using cardiotoxin injection and monitored cell proliferation and myogenic regulatory factor expression in myogenic precursors cells and muscle fibers after injury using proliferation markers and the transgenic lines. We also analyzed Pax7+ve cells in animals with dystrophic phenotypes and found an increased number compared with wild-type.
Genes / Markers
Figures
Figure Gallery (10 images)
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Expression
Phenotype
No data available
Mutations / Transgenics
Allele Construct Type Affected Genomic Region
i124TgTransgenic Insertion
    i131TgTransgenic Insertion
      i150TgTransgenic Insertion
        i167TgTransgenic Insertion
          smb237EtTransgenic Insertion
            ta222a
              Point Mutation
              y1TgTransgenic Insertion
                1 - 7 of 7
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                Human Disease / Model
                No data available
                Sequence Targeting Reagents
                Target Reagent Reagent Type
                itga7MO3-itga7MRPHLNO
                myod1MO1-myod1MRPHLNO
                1 - 2 of 2
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                Fish
                Antibodies
                Name Type Antigen Genes Isotypes Host Organism
                Ab1-actnmonoclonal
                  IgG1Mouse
                  Ab1-paxmonoclonal
                    Mouse
                    Ab2-myod1polyclonalRabbit
                    Ab2-Prox1polyclonalRabbit
                    Ab-Pax7monoclonal
                      IgG1Mouse
                      1 - 5 of 5
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                      Orthology
                      No data available
                      Engineered Foreign Genes
                      Marker Marker Type Name
                      EGFPEFGEGFP
                      TomatoEFGTomato
                      YFPEFGYFP
                      1 - 3 of 3
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                      Mapping
                      No data available