PUBLICATION

A membrane-bound vertebrate globin

Authors
Blank, M., Wollberg, J., Gerlach, F., Reimann, K., Roesner, A., Hankeln, T., Fago, A., Weber, R.E., and Burmester, T.
ID
ZDB-PUB-111012-1
Date
2011
Source
PLoS One   6(9): e25292 (Journal)
Registered Authors
Keywords
Globins, Zebrafish, Cell membranes, Vertebrates, Heme, Eyes, Myristoylation, Recombinant proteins
MeSH Terms
  • Molecular Sequence Data
  • Subcellular Fractions
  • Zebrafish/physiology*
  • Amino Acid Sequence
  • Gene Expression Regulation, Developmental
  • Lipoylation
  • Globins/genetics
  • Globins/immunology
  • Globins/metabolism*
  • Heme/metabolism
  • Oxygen/metabolism*
  • Cell Membrane/metabolism*
  • Recombinant Proteins/genetics
  • Recombinant Proteins/isolation & purification
  • Recombinant Proteins/metabolism*
  • Animals
  • Immunoglobulin G/immunology
  • Immunoglobulin G/metabolism
  • Sequence Homology, Amino Acid
  • Rabbits
  • Green Fluorescent Proteins/genetics
  • Green Fluorescent Proteins/metabolism
(all 22)
PubMed
21949889 Full text @ PLoS One
Abstract

The family of vertebrate globins includes hemoglobin, myoglobin, and other O2-binding proteins of yet unclear functions. Among these, globin X is restricted to fish and amphibians. Zebrafish (Danio rerio) globin X is expressed at low levels in neurons of the central nervous system and appears to be associated with the sensory system. The protein harbors a unique N-terminal extension with putative N-myristoylation and S-palmitoylation sites, suggesting membrane-association. Intracellular localization and transport of globin X was studied in 3T3 cells employing green fluorescence protein fusion constructs. Both myristoylation and palmitoylation sites are required for correct targeting and membrane localization of globin X. To the best of our knowledge, this is the first time that a vertebrate globin has been identified as component of the cell membrane. Globin X has a hexacoordinate binding scheme and displays cooperative O2 binding with a variable affinity (P50~1.3–12.5 torr), depending on buffer conditions. A respiratory function of globin X is unlikely, but analogous to some prokaryotic membrane-globins it may either protect the lipids in cell membrane from oxidation or may act as a redox-sensing or signaling protein.

Genes / Markers
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Expression
Phenotype
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Sequence Targeting Reagents
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Fish
Fish
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Antibodies
Name Type Antigen Genes Isotypes Host Organism
Ab1-xgbpolyclonalRabbit
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Orthology
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Engineered Foreign Genes
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Mapping
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