PUBLICATION

Motoneurons are essential for vascular pathfinding

Authors
Lim, A.H., Suli, A., Yaniv, K., Weinstein, B., Li, D.Y., and Chien, C.B.
ID
ZDB-PUB-110811-31
Date
2011
Source
Development (Cambridge, England)   138(17): 3847-3857 (Journal)
Registered Authors
Chien, Chi-Bin, Lim, Amy, Suli, Arminda, Weinstein, Brant M., Yaniv, Karina
Keywords
netrin, DCC, lymphatic, parachordal chain, motoneuron, axon guidance, vascular guidance, angiogenesis, zebrafish
MeSH Terms
  • Animals
  • In Situ Hybridization
  • Zebrafish Proteins/genetics
  • Zebrafish Proteins/metabolism*
  • Motor Neurons/cytology
  • Motor Neurons/metabolism*
  • Zebrafish
  • Nerve Growth Factors/genetics
  • Nerve Growth Factors/metabolism*
(all 9)
PubMed
21828101 Full text @ Development
Abstract

The neural and vascular systems share common guidance cues that have direct and independent signaling effects on nerves and endothelial cells. Here, we show that zebrafish Netrin 1a directs Dcc-mediated axon guidance of motoneurons and that this neural guidance function is essential for lymphangiogenesis. Specifically, Netrin 1a secreted by the muscle pioneers at the horizontal myoseptum (HMS) is required for the sprouting of dcc-expressing rostral primary motoneuron (RoP) axons and neighboring axons along the HMS, adjacent to the future trajectory of the parachordal chain (PAC). These axons are required for the formation of the PAC and, subsequently, the thoracic duct. The failure to form the PAC in netrin 1a or dcc morphants is phenocopied by laser ablation of motoneurons and is rescued both by cellular transplants and overexpression of dcc mRNA. These results provide a definitive example of the requirement of axons in endothelial guidance leading to the parallel patterning of nerves and vessels in vivo.

Genes / Markers
Figures
Figure Gallery (13 images) / 2
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Expression
Phenotype
Mutations / Transgenics
Allele Construct Type Affected Genomic Region
ml2TgTransgenic Insertion
    p151TgTransgenic Insertion
      sa29
        Point Mutation
        um13TgTransgenic Insertion
          y1TgTransgenic Insertion
            y10
              Point Mutation
              1 - 6 of 6
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              Human Disease / Model
              No data available
              Sequence Targeting Reagents
              Target Reagent Reagent Type
              dccMO1-dccMRPHLNO
              isl1aMO2-isl1aMRPHLNO
              isl1aMO3-isl1aMRPHLNO
              mttpMO1-mttpMRPHLNO
              ntn1aMO2-ntn1aMRPHLNO
              olig2MO1-olig2MRPHLNO
              plcg1MO3-plcg1MRPHLNO
              plekha7aMO1-plekha7aMRPHLNO
              robo4MO1-robo4MRPHLNO
              tnnt2aMO1-tnnt2aMRPHLNO
              1 - 10 of 10
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              Fish
              Antibodies
              Orthology
              No data available
              Engineered Foreign Genes
              Marker Marker Type Name
              DsRedxEFGDsRedx
              EGFPEFGEGFP
              GFPEFGGFP
              1 - 3 of 3
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              Mapping
              No data available